RNA干扰血管内皮生长因子受体2基因表达提高结肠癌细胞对奥沙利铂敏感性的研究

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Abstract Objective To study the inhibitory effect of RNA interference in the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) for the growth of human colon cancer cells and the chemosensitive effect of Oxaliplatin. Methods The expression plasmid of small interferon RNA (siRNA) targeting VEGFR-2 gene was constructed and human colon cancer cell line SW480 was transfected. The effect of VEGFR-2 siRNA for the expression of human colon cancer cells SW480 VEGFR-2 mRNA and protein was detected by RT-PCR and Western blot. The effect of siRNA or combining with Oxaliplatin for the cell proliferation and apoptosis was detected by MTT assay and flow cytometry. Results The mRNA and protein expression of VEGFR-2 were significantly down-regulated by VEGFR-2 siRNA (P ＜ 0.05). The combination of VEGFR-2 siRNA and Oxaliplatin could slowdown the growth of colon cancer cells, inhibit the proliferation, and significantly increase the inhibition of cell proliferation and apoptosis (P ＜ 0.05). Conclusion Silencing VEGFR-2 gene by the siRNA technology can inhibit the proliferation of SW480 cells, induce the apoptosis of SW480 cell, and enhance the chemotherapy sensitivity of Oxaliplatin for human colon cancer cells.

Key words： Colon cancer Vascular endothelial growth factor receptor-2 Small interferon RNA Oxaliplatin

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	YANG Ping WEI Jianchang CHEN Huacui ZHANG Tong ZENG Shanqi CAO Jie

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YANG Ping WEI Jianchang CHEN Huacui ZHANG Tong ZENG Shanqi CAO Jie. Study on the sensitivity of colon cancer cells for Oxaliplatin enhanced by RNA interference in the expression of vascular endothelial growth factor receptor-2[J]. 中国医药导报, 2017, 14(20): 16-19.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I20/16

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