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| Effects of Ziyin Mingmu Pills on the expression of Fas/FasL in the retina of RCS rats |
| WANG Ying1,2 JIANG Pengfei1,2 PAN Kun1,2 PENG Jun2,3 XU Jian1,2 PENG Qinghua1,2,3 |
1.College of Traditional Chinese Medicine, Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410208, China;
2.Hu′nan Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases, Hu′nan Province, Changsha 410208, China;
3.Department of Ophthalmology, the First Affiliated Hospital of Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410007, China |
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Abstract Objective To observe the effect of Ziyin Mingmu Pills on the expression of Fas/FasL in the retina of RCS rats. Methods The RCS rats were selected, aged (1.51±0.27) months, total 24 rats, they were randomly divided into 3 groups: blank group, model group, Ziyin Mingmu Pills group (4 male and 4 female, n = 8). Blank group: RCS (rdy+/+, p+/+) rats were given normal saline by intragastric administration; model group: RCS (rdy-/-, p-/-) rats were given normal saline by intragastric administration; Ziyin Mingmu Pills group: RCS (rdy-/-, p-/-) rats were given Ziyin Mingmu Pills by intragastric administration. After intragastric administration for 30 d, the morphological changes of each layer of the retina sample were observed by immunohistochemistry. The expression levels of Fas/FasL in the retina of rats were determined by Western blot. Results The results of immunohistochemistry showed that each layer of retinal structure of the rats in the blank group was clear; the thickness of retina in the model group was lower than that in the blank group and the structure of each layer of retina was not clear; the retinal thickness of Ziyin Mingmu Pills group was lower than that of blank group but higher than that of blank group, the structure of each layer of retina was clear. The results of Western blot assay showed that the relative expression of Fas, FasL protein in the retina of Ziyin Mingmu Pills group was lower than that in the model group, the difference was highly statistically significant (P ＜ 0.01). Conclusion Ziyin Mingmu Pills has a protective effect on the ultrastructure of retina, which can inhibit the expression of Fas/FasL of the retina and reduce the apoptosis of retinal photoreceptor cells, thus achieving the purpose of protecting visual cells.
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