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| Effects of water extract of berberine on apoptosis and proliferation of human skin tumor A431 cells |
| GAO Yaoxing1 YU Jianshe1 DU Rina2 YANG Limin2 ZHAO Pengwei3 SUN Peng3 |
1.Department of Anesthesiology, Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia Autonomous Region, Hohhot 010010, China;
2.Department of Dermatology, International Mongolian Medical Hospital of Inner Mongolia Autonomous Region, Inner Mongolia Autonomous Region, Hohhot 010010, China;
3.Inner Mongolia Medical University, Inner Mongolia Autonomous Region, Hohhot 010010, China |
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Abstract Objective To investigate the effect of water extract of berberine on apoptosis and proliferation of human skin tumor A431 cells. Methods The low temperature extraction process was used to extract the water-soluble small molecule mixture from the medicinal materials. MTT assay was used to detect the inhibitory effect of the drug on human skin tumor A431 cells. Four concentrations of 6.3, 12.5, 25, 50 μg/μL were set as the drug group, and those without the drug were set as the control group. The tumor inhibition rates at 24, 48 and 72 h were observed. Tunel was used to detect apoptosis. After being screened by MTT, the apoptosis pathways of caspase-3, caspase-8, caspase-9 and Bcl-2 were detected by Western blot after treating tumor A431 cells with 25 μg/μL water extract for 48 h. Results The main components were nucleic acid, alkaloid, etc. MTT showed that the inhibition rate of 25 μg/μL water extract of berberine on A431 cells reached the best at 48 h (P ＜ 0.05). Under the microscope, there was no change in the control group. After incubated with 25 μg/μL water extract of berberine for 48 h, the number of cells significantly decreased, became round and floated. Tunel showed 25 μg/μL water extract of berberine significantly increased green light at the approved location, indicating a large number of apoptosis. In this study, after incubated with 25 μg/μL water extract of berberine for 48 h, and the expressions of caspase-3, caspase-8 and caspase-9 in tumor A431 cells were significantly increased, while the expression of Bcl-2 was significantly decreased. Conclusion This study preliminarily showed that the small molecule mixture of water extract from rhizoma coptiliae could promote apoptosis of A431 cells and inhibit cell proliferation. The mechanisms include the extracellular apoptosis pathway and the intracellular apoptosis pathway, leading to the up-regulation of caspase-3, caspase-8 and caspase-9 in tumor cells and the down-regulation of the apoptotic protein Bcl-2. This study further clarified the mechanism of wat extract of berberine in the treatment of skin tumors and provided methods for the treatment of skin tumors.
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