支原体肺炎合并细菌感染患儿T细胞亚群及降钙素原检测的诊断能效

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Abstract Objective To explore the valve of detection of T cell subsets and procalcitonin (PCT) in children with Mycoplasma pneumoniae pneumonia (MPP) combined with bacterial infection. Methods A total of 106 children with MPP was recruited from October 2016 to March 2018 in Department of Pediatrics, Jianyang People′s Hospital of Sichuan Province ("our hospital" for short), and were divided into MPP group (41 cases) and bacterial infection group (MPP combined with bacterial infection, 65 cases) according to pathogeny examination, 50 cases of healthy children were recruited as control group. The blood were collected to count CD4+T cells, CD8+T cells by fluorescence activated cell sorter, and PCT, C reactive protein (CRP), white blood cell (WBC) were detected. Results PCT, CRP and WBC in bacterial infection group were significantly higher than those in control group and MPP group (P < 0.05), and CRP in MPP group was significantly higher than that in control group (P < 0.05). CD4+T cells in bacterial infection group and MPP group were significantly lower than those in control group, CD4+T cells in bacterial infection group were lower than those in MPP group (P < 0.05). CD8+T cells in bacterial infection group and MPP group were significantly higher than those in control group (P < 0.05). CD4+/CD8+ in bacterial infection group and MPP group were significantly lower than those in control group, CD4+/CD8+ in bacterial infection group was lower than that in MPP group (P < 0.05). ROC analysis showed that PCT and T cell subsets had a higher diagnostic value for bacterial infections, of which PCT AUC was more than 0.8. Conclusion CD4+/CD8+ reducted and serum PCT concentration elevated in children with MPP combined with bacterial infection. The detection of T cell subsets and PCT is of great significance in identifying whether MPP patients combined with bacterial infection. With the help of other auxiliary examinations, it can pricisely judge disease and reduce the abuse of antibiotics.

Key words： Mycoplasma pneumoniae pneumonia Bacterial infection T cell subsets Procalcitonin

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	WANG Pingkang LI Lixue ZHANG Xiang WANG Guixiang

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WANG Pingkang LI Lixue ZHANG Xiang WANG Guixiang. Diagnostic energy efficiency of T cell subsets and procalcitonin in children with Mycoplasma pneumoniae pneumonia combined with bacterial infection[J]. 中国医药导报, 2019, 16(15): 81-84.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I15/81

[1] Liu CL，Wang GQ，Zhang B，et al. Mycoplasma pneumoniae pneumonia in hospitalized children diagnosed at acute stage by paired sera [J]. Chin Med J （Engl），2010，123（23）：3444-3450.
[2] 陆月明，王丽娜，顾秀莲.血清降钙素原对社区获得性肺炎诱发急性呼吸窘迫综合征患者预后的预测价值[J].中国急救医学，2009，29（10）：929-931.
[3] 殷勇，陆权，闫晓莉，等.肺炎支原体感染的流行病学[J].中华儿科杂志，2016，54（2）：196-197.
[4] Upadhyay S，Niederman MS. Biomarkers：what is their benefit in the identification of infection， severity assessment，and management of community-acquired pneumonia? [J]. Infect Dis Clin North Am，2013，27（1）：19-31.
[5] Assicot M，Gendrel D，Carsin H，et al. High serum procalcitonin concentrations in patients with sepsis and infection [J]. Lancet，1993，341（8844）：515.
[6] 郑秀芬，许亚茹，王岩，等.儿科常见急性呼吸道感染疾病超敏C-反应蛋白检测结果分析[J].中国煤炭工业医学杂志，2015，18（7）：1104-1107.
[7] 胡亚美.诸福棠实用儿科学[M].北京：人民卫生出版社，2002.
[8] Ngeow YF，Suwanjutha S，Chantarojanasriri T，et al. An Asian study on the prevalence of atypical respiratory pathogens in community-acquired pneumonia [J]. Int J Infect Dis，2005，9（3）：144-153.
[9] 谢娜，陈志敏.肺炎支原体感染儿童血清免疫相关指标的变化及临床意义[J].中国现代医生，2014，52（8）：104-107.
[10] 曹春月，马丽，陈慧娟.肺炎支原体抗体检测的两种方法学比较[J].检验医学与临床，2018，15（20）：3030-3032.
[11] Wang X，Chen X，Tang H，et al. Increased frequency of Th17 cells in children with Mycoplasma pneumoniae pneumonia [J]. J Clin Lab Anal，2016，30（6）：1214-1219.
[12] Miller AC，Rashid RM，Elamin EM. The "T" in trauma：the helper T-cell response and the role of immunomodulation in trauma and burn patients [J]. J Trauma，2007， 63（6）：1407-1417.
[13] Atkinson TP，Duffy LB，Pendley D，et al. Deficient immune response to Mycoplasma pneumoniae in childhood asthma [J]. Allergy Asthma Proc，2009，30（2）：158-165.
[14] Gurin S. Evaluation of the detection of procalcitonin by an immuno-chromatography test：Brahms PCT-Q [J]. Ann Biol Clin，2000，58（5）：613-614.
[15] 李娇.肺炎支原体抗体与血清总IgE检验在诊断小儿肺炎中的应用效果观察[J].中国实用医药，2018，13（30）：46-47.
[16] 解玲玲，孙亚娜，吴良霞.肺炎支原体肺炎合并Epstein-Barr病毒感染患儿的血清因子及免疫分析[J].微生物与感染，2018，13（5）：278-283.
[17] 吴勤如，何惠玲，蒋英.C-反应蛋白检测在儿童肺炎诊断中的临床应用[J].国际检验医学杂志，2005，26（3）：177-178.
[18] 李治君.肺炎支原体抗体联合超敏C反应蛋白检测对小儿支原体肺炎的诊断价值[J].广西医科大学学报，2018， 35（9）：1267-1269.
[19] 李晓，周春菊，邓燕妮，等.肺炎支原体抗体、C-反应蛋白及血清降钙素原检测在儿童呼吸道感染诊断中的应用评价[J].现代诊断与治疗，2018，29（14）：2261-2263.
[20] Trautner BW，Caviness AC，Gerlacher GR，et al. Prospective evaluation of the risk of serious bacterial infection in children who present to the emergency department with hyperpyrexia （temperature of 106°F or higher） [J]. Pediatrics，2006，118（1）：34-40.
[21] Ding Y，Chu C，Li Y，et al. High expression of HMGB1 in children with refractory Mycoplasma pneumoniae pneumonia [J]. BMC Infect Dis，2018，18（1）：439.