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| The characteristics of rat retinal Müller cells in high glucose, oxidative stress and hypoxia |
| LI Chunhua1 FENG Zhaohui2 ZHANG Xue1 TIAN Bingyu1 LEI Xiaoqin1 ZHOU Tingjie3 MA Weimei1 |
1.Department of Ophthalmology, Xi′an No.4 Hospital, Shaanxi Province, Xi′an 710004, China;
2.Department of Ophthalmology, the Second Affiliated Hospital of Xi′an Jiaotong University Medical College, Shaanxi Province, Xi′an 710004, China;
3.Xi′an Center for Disease Control and Prevention, Shaanxi Province, Xi′an 710054, China |
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Abstract Objective To observe the characteristics of Sprague-Dawley (SD) rats retinal Müller cells under three pathological conditions including high glucose, oxidative stress and hypoxia, and to provide new research basis for the prevention and treatment of diabetic retinopathy. Methods SD rat retinal Müller cells were cultured in vitro and identified by glutathione synthetase (GS). The second generation of cells were divided into control group, high glucose group, oxidative stress group and hypoxia group randomly. The morphological changes of the cells were observed under inverted microscope, the changes of GS and α-smooth muscle actin (α-SMA) were observed by immunofluorescence staining, and the cell migration ability was observed by Transwell chamber. Results The Müller cells were successfully cultured and identified in vitro. In all groups, the function of Müller cells was impaired, and in oxidative stress group and hypoxia group, the structure was damaged, and the normal morphology was lost. In hypoxia group, the expression of α-SMA was positive, and the number of cells in the Transwell chamber was significantly higher than that in other groups, the differences were statistically significant (P ＜ 0.05). Conclusion High glucose, oxidative stress and hypoxia can destroy the function of Müller cells. Hypoxia can induce transforming into myofibroblast cells with migratory ability, which indicates that Müller cells are involved in the pathogenesis of proliferative diabetic retinopathy.
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