蛴螬提取物对实验性兔视网膜静脉阻塞不同时间窗MMP-9表达的影响

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Abstract Objective To evaluate the therapeutic effect and mechanism of white grub extract on experimental rabbits with retinal vein occlusion (RVO) in different time windows. Methods Forty rabbits were randomly divided into 4 groups: group A, group B, group C, group D, with 10 rabbits and 20 eyes in each group. Except for group A, photochemical kinetic method was used to establish RVO animal model in the other three groups. Group A and B were intragastrically administered with 5 mL/kg of normal saline. Group C was intragastrically administered with 5 mL/kg of Compound Xueshuantong Suspension, and group D was intragastrically administered with 1.5 mL/kg of white grub extract. After administration for 1 and 3 weeks, fluorescein fundus angiography (FFA) was examined to calculate to the ratio of area of non-perfusion area and area of optic disk, HE staining was used to observe the retinal morphology, immunohistochemistry was used to detect the expression of matrix metalloproteinase (MMP)-9. Results The results of FFA test showed that the ratio of area of non-perfusion area and area of optic disk in group C and D was significantly lower than that of group B after modeling for 1 week and 3 weeks, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The results of light microscopy of retinal sections showed that in group B, 1 week after modeling, the retinal tissue was edematous, the cells were arranged in disorder, and there was no new vascular cord, after 3 weeks of modeling, a large number of neovascular cords appeared; in group C, 1 week after modeling, the retinal tissue was edematous, the cells were arranged in disorder, 3 weeks after modeling, the edema of retinal tissue was reduced, there was no neovascular cord; in group D, 1 week after modeling, the retinal tissue cells were roughly arranged, 3 weeks after modeling, the edema of retinal tissue was reduced, there was no neovascular cord. The results of MMP-9 immunohistochemistry showed that the expression of MMP-9 was up-regulated in each group, group D was the weakest, followed by group C, and group B was the strongest, the expression of MMP-9 in group B, C and D was higher than that of group A, while the expression of MMP-9 in group C and D was lower than that of group B, the differences were all statistically significant (P ＜ 0.05). Conclusion White grub extract can improve the state of retinal ischemia and hypoxia and inhibit the expression of MMP-9 in the experimental RVO model.

Key words： Retinal vein occlusion White grub extract Matrix metalloproteinase-9 Neovascular

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	JIANG Pengfei1
	2 MA Junxu1
	2 PENG Jun2
	3 PENG Qinghua1
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JIANG Pengfei1,2 MA Junxu1,2 PENG Jun2, et al. Effect of white grub extract on the expression of MMP-9 of experimental rabbits with retinal vein occlusion in different time windows[J]. 中国医药导报, 2019, 16(15): 8-11.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I15/8

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