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| A model of delayed encephalopathy acute carbon monoxide poisoning established with inhalation method |
| CHEN Chao1 WANG Baojun2 XIANG Wenping2 LI Yuechun2 HAO Xiwa2 PANG Jiangxia2 GAO Haoran2 XIE Wei3 |
1.Department of Neurology, Center Hospital of Baotou, Inner Mongolia Medical University, Inner Mongolia Autonomous Region, Baotou 014000, China;
2.Department of Neurology, Center Hospital of Baotou, Inner Mongolia Autonomous Region, Baotou 014000, China;
3.Central Laboratory, Baotou Medical College, Inner Mongolia Autonomous Region, Baotou 014000, China |
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Abstract Objective To establish a model of delayed encephalopathy acute carbon monoxide poisoning (DEACMP). Methods A total of 196 intelligence-qualified rats were divided into experimental group and control group by random number table method, with 164 rats in the experimental group and 32 rats in the control group. The experimental group used an acute static inhalation of carbon monoxide (CO) to establish a poisoned rat model. According to different concentrations of CO poisoning, they were randomly divided into 1000, 3000, 4000 ppm group, with 32 rats alive in each group. Each group continued to inhale for 40 minutes. Rats in each group were sacrificed at 7, 14, 21 and 28 d after poisoning. Spectrophotometer method was used to dynamically monitor the changes of carboxyhemoglobin (COHb) content in each group. Morris water maze test was used to evaluate cognitive function. HE staining was used to observe the cell damage in hippocampus. The best poisoning concentration and time for establishing a DEACMP rat model was established. Results After poisoned by CO, the rats showed typical symptoms of poisoning. There was a significant positive correlation between COHb content and CO concentration (r = 0.748, P ＜ 0.05). The mortality rates of the 1000, 3000, 4000 ppm group and the control group were 0%, 40.74%, 58.97% and 0%, respectively. The incidence of DEACMP in each group was 0%, 6.25%, 28.13%, 31.25%, and 0%, and the 21 d and 28 d after being poisoned were high DEACMP time points. Based on the low mortality rate and high incidence rate, the CO poisoning concentration of 3000 ppm and the time of 21d were the best method to establish a DEACMP rat model. Conclusion A DEACMP rat model can be established with a static inhalation concentration of 3000 ppm CO for 40 minutes, and models can be successfully established 21 days after poisoning.
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