葡萄糖调节蛋白GRP78在小梁网细胞中的表达及临床意义

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Abstract [Abstract] Objective To detect protein and mRNA expression differences of GRP78 in human trabecular meshwork cells of normal eyes (NTM) and POAG patients (GTM) in vitro. Methods Human trabecular meshwork cells from NTM and GTM in vitro were divided into 3 groups: Tunicamycin (Tm) group, Staurosporine (STS) group and control group. Real-time RT-PCR and Western blot were used to detect the expression of mRNA and protein of GRP78 in trabecular meshwork cells after 6, 24 h treatment. Results The expression of GRP78 in primary culture GTM cells was significantly lower than that in NTM cells, with statistically significant difference (P < 0.05). Tm could increase the expression of GRP78 in NTM and GTM cells, the expression in GTM cells was lower than that in NTM cells, with statistically significant difference (P < 0.05). The response of GTM cells for STS was bigger than NTM cells. Conclusion GTMs showed insensitivity reactions when stimulated with inducer of endoplasmic reticulum stress, reduce the expression of GRP78, lead to the increased sensitivity of GTMs to damage stimulus. GRP78 may play a cytoprotective role in the apoptosis in trabecular meshwork cells caused by endoplasmic reticulum stress.

Key words： [Key words] Glaucoma Trabecular meshwork cells Endoplasmic reticulum stress

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	CHAI Fang1 ZHUO Yehong2 YANG Xinguang1▲

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CHAI Fang1 ZHUO Yehong2 YANG Xinguang1▲. Expression and clinical significance of GRP78 in human trabecular meshwork cells[J]. 中国医药导报, 2016, 13(3): 114-117.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2016/V13/I3/114

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