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| Effect of Genistein on FSHR gene expression in ovarian granulosa cells of female rats |
| WEN Haixia ZHANG Ya′nan |
| Department of Physiology, Basic Medical College, Harbin Medical University, Heilongjiang Province, Harbin 150086, China |
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Abstract Objective To observe the effect of Genistein on the expression of follicle stimulating hormone receptor (FSHR) gene in ovarian granulosa cells of female rats and its relationship with estrogen receptor (ER). Methods Female rats aged 25-28 days were subcutaneously injected with pregnant mare serum gonadotrophin to establish follicular development model. Ovarian granulosa cells were isolated and cultured. Genistein (0, 0.1, 1,5, 10, 100 μmol/L) and estrogen receptor blocker ICI 182,780 (1 μmol/L) were treated for 24 hours respectively. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of FSHR mRNA, an upstream regulator of aromatase gene. Results Compared with the blank control group, the expression of FSHR mRNA was significantly increased in the 10 μmol/L Genistein group (P < 0.05). After ICI 182,780 (1 μmol/L) pretreatment for 30 minutes, the expression of FSHR in ICI 182,780 pretreated cells with 1 and 10 μmol/L Genistein was significantly lower than that in the 1 and 10 μmol/L Genistein groups (P < 0.05 or P < 0.01) respectively. Conclusion 10 μmol/L of Genistein can up-regulate the expression of FSHR in ovarian granulosa cells of female rats, and this effect may be mediated by ER.
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