枸杞丹参对视网膜色素变性大鼠视网膜组织形态学及CRYAB mRNA的影响

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Abstract Objective To observe the effects of Lycium barbarum and Salvia miltiorrhiza on the morphology of retinal tissue and alpha B-crystalline (CRYAB) mRNA in rats with retinitis pigmentosa. Methods Twenty-four male and female retinitis pigmentosa RCS (rdy-/-, p-/-) rats were divided into a model group and a ginseng group according to random number table method, with 8 rats in each group, 4 males and 4 females. Another 8 RCS (rdy+/+, p+/+) rats, 4 males and 4 females, were blank group. Oral gavage was started 1 week after routine feeding, once a day. The blank group and the model group were given normal saline, and the ginseng group was given an equivalent dose of Lycium barbarum + Salvia miltiorrhiza. The rats were sacrificed for 30 days after continuous gavage. The morphological changes of the outer nuclear layer of the retina of each group were observed under light microscope. The expression of CRYAB mRNA in RCS rats was detected by qPCR. Results The results of HE staining showed that the retinal structure of each layer in the blank group was neatly arranged and regular in shape. The retinal structure of the model group was looser, with degeneration and atrophy, and the thickness of the outer nuclear layer was significantly thinner, the difference was highly statistically significant (P ＜ 0.01). Compared with the blank group, the ginseng group had a disordered structure and thinner thickness, but it had increased thickness compared with the model group, and the difference was highly statistically significant (P ＜ 0.01). The results of qPCR showed that the expression of CRYAB mRNA was observed in the retina of rats in the blank group. The expression of CRYAB mRNA in the retina of the model group was significantly decreased compared with the blank group (P ＜ 0.01). The expression of CRYAB mRNA in the retina of the ginseng group was higher than that in the model group and the difference was highly statistically significant (P ＜ 0.01). Conclusion RCS rats can well simulate the pathogenesis of human retinitis pigmentosa. The structure of the retina is disordered, the outer nuclear layer is thinned, the expression of CRYAB mRNA in the retina is inhibited, and Lycium barbarum + Salvia miltiorrhiza can induce the expression of CRYAB mRNA in the retina. It has a certain protective effect on the retina of retinitis pigmentosa RCS rats.

Key words： Retinitis pigmentosa Lycium barbarum Salvia miltiorrhiza CRYAB mRNA

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	LIU Jiaqi1 WANG Ying1 JIANG Pengfei1 PAN Kun1 XU Jian1 PENG Jun2
	3 PENG Qinghua1
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LIU Jiaqi1 WANG Ying1 JIANG Pengfei1 PAN Kun1 XU Jian1 PENG Jun2,3 PENG Qinghua1,2, et al. The effects of Lycium barbarum and Salvia miltiorrhiza on the histomorphology and CRYAB mRNA of retinal pigment degeneration rats[J]. 中国医药导报, 2019, 16(14): 13-15,22.

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