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| Effects of pepper acid ethylenediamine on blood lipid levels and antioxidant capacity in hyperlipidemia rats |
| ZHANG Rui1 Tuersunbaike·Yeerda2 LYU Meixia1 Dina·Muheyati1 Ayiguli·Alimu1 PENG Bingxin1 Wubulikasimu·Aikebaier1 HAN Jia1 |
| 1.Department of Nutrition and Food Hygiene, College of Public Health, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China; 2.College of Chemistry and Biological Sciences, Yili Normal University, Xinjiang Uygur Autonomous Region, Yining 835000, China |
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Abstract Objective To study the effects of pepper acid ethylenediamine (PAE) on blood lipid levels and antioxidant capacity in hyperlipidemia rats. Methods Sixty-five SPF male Sprague-Dawley rats were selected. Their basic serum cholesterol (TC) and triglyceride (TG) were determined. 10 rats were selected as the normal control group and given normal diets. 55 rats were fed with high-fat diet as model groups. After 4 weeks of intervention, TC and TG were detected again. Compared with the blood lipid levels before and after modeling, TC and TG were significantly increased, then the model was believed to have been built successfully. The 50 rats with successful modeling were divided into 5 groups: high-fat model group (n = 10), positive drug group (Simvastatin, 10 mg/kg, n = 10), and high (10 mg/kg, n = 10), medium (5 mg/kg, n = 10) and low (2.5 mg/kg, n = 10) dose of PAE groups. Each group was continuously intragastrically administered for 4 weeks, once a day. At the end of the fourth week, serum was collected for analysis of the levels of TC, TG, high density lipoprotein (HDL-C), low density lipoprotein (LDL-C), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). Results Compared with the normal control group and hyperlipidemia model group, each dose of PAE groups could reduce the levels of TC, TG, LDL-C and raise HDL-C (P ＜ 0.05 or P < 0.01); compared with the positive drug group, the levels of LDL-C in high dose of PAE group were decreased (P ＜ 0.05). Compared with the high fat model group, the activity of T-SOD and GSH-Px in high dose of PAE group increased (P ＜ 0.05 or P < 0.01), the content of MDA in positive drug group and middle dose of PAE group was significantly decreased (P ＜ 0.01). Compared with the positive drug group, the content of MDA in high dose of PAE group inecreased (P ＜ 0.05). Conclusion PAE can reduce blood lipid levels, increase the activity of serum T-SOD, GSH-Px, and decrease MDA content in hyperlipidemia rats, suggesting that the hypolipidemic effect of PAE may be ralated to improving oxidative stress in rats with high fat diet.
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