椎间隙感染后持续腰背痛的机制研究

Abstract
Figure/Table
References (23)
Related Citation (4)

Download: PDF (710 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To Discus on the Staphylococcus aureus supernatant of intervertebral disc nucleus pulposus cells proteoglycans (ACAN), type Ⅱ collagen (COL Ⅱ). Methods Cultured SD rat intervertebral disc nucleus pulposus cells were isolated in vitro and treated with Staphylococcus aureus supernatant at different concentrations (0%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%) to simulate the course of intervertebral space infection. CCK-8 was used to detect the proliferation activity of the second generation nucleus pulposus (P2) cells. RT-PCR was used to detect the gene expression of the second generation nucleus pulposus cells. Results This study found that the supernatant fluid of Staphylococcus aureus that accelerate nucleus pulposus cells to divide, stimulate ACAN, COL Ⅱ expression decreased (P ＜ 0.05), the supernatant fluid concentration > 20%, can significantly inhibit extracellular matrix synthesis of nucleus pulposus. Conclusion The results showed that Staphylococcus aureus supernatant could inhibit the growth of nucleus pulposus cells in a concentration dependent manner. Stimulate ACAN, COL Ⅱ expression decrease may be the cause of intervertebral infection after continuous low back pain.

Key words： Nucleus pulposus Spontaneous spondylodiscitis Low back pain Staphylococcus aureus

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	YANG Huan1 SONG Hongxing2▲ CUI Lei3 YUAN Wei2

Cite this article:

YANG Huan1 SONG Hongxing2▲ CUI Lei3 YUAN Wei2. Mechanism of persistent low back pain after intervertebral space infection[J]. 中国医药导报, 2019, 16(13): 8-11.

URL:

http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I13/8

[1] Fantoni M，Trecarichi EM，Rossi B，et al. Epidemiological and clinical features of pyogenic spondylodiscitis [J]. Eur Rev Med Pharmacol Sci，2012，16（S2）：2-7.
[2] Boody BS，Tarazona DA，Vaccaro AR. Evaluation and Management of Pyogenic and Tubercular Spine Infections [J]. Cur Rev in Musculoskelet Med，2018，11（4）：643-652.
[3] Tyagi R. Spinal infections in children：A review [J]. J Orthopaedics，2016，13（4）：254-258.
[4] Gruss P，Tannenbaum H，Ott-Tannenbaum B，et al. [Follow-up of spondylodiscitis following intervertebral disk operation-on the etiology，therapy and prevention] [J]. Neurochirurgia （Stuttg），1992，35（3）：79-84.
[5] Mylona E，Samarkos M，Kakalou E，et al. Pyogenic vertebral osteomyelitis：a systematic review of clinical characteristics [J]. Semin Arthritis Rheum，2009，39（1）：10-17.
[6] Ratcliffe JF. Anatomic basis for the pathogenesis and radiologic features of vertebral osteomyelitis and its differentiation from childhood discitis. A microarteriographic investigation [J]. Acta Radiol Diagn （Stockh），1985，26（2）：137-143.
[7] Widdrington J，Emmerson I，Cullinan M，et al. Pyogenic Spondylodiscitis：Risk Factors for Adverse Clinical Outcome in Routine Clinical Practice [J]. Medical Sciences，2018，6（4）：96.
[8] D′Agostino C，Scorzolini L，Massetti AP，et al. A seven-year prospective study on spondylodiscitis：epidemiological and microbiological features [J]. Infection，2010，38（2）：102-107.
[9] Arko LT，Quach E，Nguyen V，et al. Medical and surgical management of spinal epidural abscess：a systematic review [J]. Neurosurg Focus，2014，37（2）：E4.
[10] Gouliouris T，Aliyu SH，Brown NM. Spondylodiscitis：update on diagnosis and management [J]. J Antimicrob Chemother，2010，65 Suppl 3：i11-i24.
[11] Freemont AJ. The cellular pathobiology of the degenerate intervertebral disc and discogenic back pain [J]. Rheumatology （Oxford），2009，48（1）：5-10.
[12] Boxberger JI，Sen S，Yerramalli CS，et al. Nucleus pulposus glycosaminoglycan content is correlated with axial mechanics in rat lumbar motion segments [J]. J Orthop Res，2006，24（9）：1906-1915.
[13] He Y，Zheng Q，Simonsen O，et al. The development and characterization of a competitive ELISA for measuring active ADAMTS-4 in a bovine cartilage ex vivo model [J]. Matrix Biol，2013，32（3/4）：143-151.
[14] Ding F，Shao ZW，Xiong LM. Cell death in intervertebral disc degeneration [J]. Apoptosis，2013，18（7）：777-785.
[15] Risbud MV，Shapiro IM. Role of cytokines in intervertebral disc degeneration：pain and disc content [J]. Nat Rev Rheumatol，2014，10（1）：44-56.
[16] Risbud MV，Schoepflin ZR，Mwale F，et al. Defining the Phenotype of Young Healthy Nucleus Pulposus Cells：Recommendations of the Spine Research Interest Group at the 2014 Annual ORS Meeting [J]. J Orthop Res，2015，33（3）：283-293.
[17] Smith LJ，Nerurkar NL，Choi KS，et al. Degeneration and regeneration of the intervertebral disc：lessons from development [J]. Dis Model Mech，2010，4（1）：31-41.
[18] Wang C，Zhang ZZ，Yang W，et al. MiR-210 facilitates ECM degradation by suppressing autophagy via silencing of ATG7 in human degenerated NP cells [J]. Biomed Pharmacother，2017，93：470-479.
[19] Tan Y，Yao X，Dai Z，et al. Bone morphogenetic protein 2 alleviated intervertebral disc degeneration through mediating the degradation of ECM and apoptosis of nucleus pulposus cells via the PI3K/Akt pathway [J]. Int J Mol Med，2019，43（1）：583-592.
[20] Pockert AJ，Richardson SM，Le Maitre CL，et al. Modified expression of the ADAMTS enzymes and tissue inhibitor of metalloproteinases 3 during human intervertebral disc degeneration [J]. Arthritis Rheum，2009，60（2）：482-491.
[21] Lv J，Li S，Wan T，et al. Inhibition of microRNA-30d attenuates the apoptosis and extracellular matrix degradation of degenerative human nucleus pulposus cells by up-regulating SOX9 [J]. Chem Biol Interact，2018，296：89-97.
[22] Medzhitov R，Horng T. Transcriptional control of the inflammatory response [J]. Nat Rev Immunol，2009，9（10）：692-703.
[23] Anderson DD，Chubinskaya S，Guilak F，et al. Post-traumatic osteoarthritis：improved understanding and opportunities for early intervention [J]. J Orthop Res，2011，29（6）：802-809.