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| Expression of CRP, IL-8 and VEGF in serum and peritoneal fluid of patients with endometriosis and its significance |
| WEI Shunying WANG Liehong QI Qingling WANG Qianyin WANG Yonghua JIA Jia |
| Department of Gynaecology, Qinghai Red Cross Hospital, Qinghai Province, Xi′ning 810000, China |
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Abstract Objective To explore the expression of C-reactive protein (CRP), interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF) in serum and peritoneal fluid of patients with endometriosis and its clinical significance. Methods A total of 93 patients with endometriosis (endometriosis group) admitted to Qinghai Red Cross Hospital from August 2015 to August 2017 were divided into stage Ⅰ (n = 19), stage Ⅱ (n = 25), stage Ⅲ (n = 28) and stage Ⅳ (n = 21) according to the American Fertility Society (r-AFS). At the same time, 60 patients with uterine leiomyoma (uterine leiomyoma group) and 60 healthy volunteers as control group were randomly selected. The levels of CRP, IL-8 and VEGF in serum and peritoneal fluid was detected by enzyme-linked immunosorbent assay. Results The levels of serum CRP, IL-8 and VEGF in endometriosis group were higher than those in uterine leiomyoma group and control group (P < 0.05). The levels of CRP, IL-8 and VEGF in peritoneal fluid were higher than those in patients with uterine leiomyoma group (P < 0.05). With the increase of disease stages, the levels of CRP, IL-8 and VEGF in serum and peritoneal fluid in patients with endometriosis increased gradually (P < 0.05). Pearson moment correlation analysis showed that serum CRP, IL-8, VEGF was positively associated with r-AFS score (r = 0.639, 0.712, 0.655; P < 0.05), and which in peritoneal fluid was positively associated with r-AFS score (r = 0.682, 0.764, 0.670; P < 0.05). The area under ROC curve (AUC) of CRP was 0.784, the best cut-off value was 12.23 mg/L, the sensitivity and specificity was 0.77 and 0.72, respectively, and the accuracy was 0.71. The AUC of IL-8 was 0.884, the best cut-off value was 20.97 mg/L, the sensitivity and specificity was 0.82 and 0.76, respectively, and the accuracy was 0.78. The AUC of VEGF was 0.889, the best cut-off value was 175.34 pg/mL, the sensitivity and specificity were 0.82 and 0.78, respectively, and the accuracy was 0.80. The AUC of CRP, IL-8 and VEGF was 0.931, the sensitivity and specificity was 0.92 and 0.85, respectively, and the accuracy was 0.89. Conclusion The levels of CRP, IL-8 and VEGF in serum and peritoneal fluid of patients with endometriosis are significantly elevated and positively associated with the progression of the disease. Early detection of CRP, IL-8 and VEGF is helpful to differential diagnosis of endometriosis and to evaluate the severity of the disease.
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