青海地区藏族、汉族HIF-2a基因单核苷酸多态性与胃癌易感性的相关性研究

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Abstract Objective To explore relationship between single nucleotide polymorphism of HIF-2Αα gene and susceptibility to gastric cancer of Qinghai area Tibetan, Han nationality. Methods A total of 51 Tibetan and 60 Han nationality patients (long lived in Qinghai area) with gastric cancer were collected. DNA was extracted from peripheral blood. Polymerase chain reaction (PCR) was used to detect 3 sites of HIF-2α gene, namely rs6715787, 13419896 and 7598371, and correlation analysis was performed. Results The SNP genotypes of rs6715787 and rs7598371 were C/G, C/C and G/G, respectively, and the SNP genotypes of rs13419896 were A/G, G/G and A/A. The genotype frequencies of A/G, G/G and A/A in rs13419896 of Tibetan and Han nationality groups were 27.45%, 29.41%, 43.13% and 45.00%, 43.33%, 11.67%, respectively. The difference in genotype frequency distribution between the two groups was highly statistically significant (χ2 = 13.518, P ＜ 0.01), and the difference in allele was highly statistically significant (χ2 = 14.195, P ＜ 0.01). The allele difference was statistically significant (χ2 = 11.495, P ＜ 0.01). The frequency distribution of allele A in Tibetan gastric cancer group was higher than that in Han nationality gastric cancer group. The C/G, C/C, G/G genotype frequencies of rs7598371 in the Tibetan and Han nationality groups were 29.42%, 37.25%, 33.33% and 41.67%, 5.00%, 53.33%, respectively. The difference of genotype frequency distribution between the two groups was highly statistically significant (χ2 = 18.118, P ＜ 0.01), and the allele differences were highly statistically significant (χ2 = 16.002, P ＜ 0.01). The frequency distribution of allele C in Tibetan nationality gastric cancer group was higher than that in Han nationality gastric cancer group. The genotype distribution of the two groups was consistent with Hardy-Weinberg equilibrium law (P < 0.05). The C/G, C/C, G/G genotype frequencies of rs6715787 in the Tibetan and Han nationality group were 35.29%, 41.18%, 23.52% and 51.67%, 8.33%, 40.00%, respectively. The frequency distribution of allele C in Tibetan nationality gastric cancer group was highly significantly Higher nationality than that in han gastric cancer group (χ2 = 16.675, P ＜ 0.01). The expression of the gene loci was different in the Tibetan and Han nationality patients with gastric cancer, among which the high expression of HIF-2α gene of C/C type in the gastric cancer patients of Tibetan population in Qinghai province was found in rs6715787. The HIF-2α gene rs7598371 of C/C type was highly expressed in Tibetan gastric cancer patients. HIF-2α gene rs6715787 of C/G type is highly expressed in gastric cancer patients of Han nationality. The HIF-2α gene rs7598371 of G/G type was highly expressed in gastric cancer patients of Han nationality. Conclusion HIF-2α gene rs7598371, rs6715787, rs13419896 gastric cancer may be no correlation between the Han nationaltiy Chinese region of Qinghai Tibetan but region of Qinghai Tibetan, Han nationaltiy gastric cancer HIF-2α gene single nucleotide polymorphisms vary, gene loci to hide the control group, Tibetan and Han nationaltiy patients with gastric cancer express different, tip region of Qinghai Tibetan, Han nationaltiy gastric cancer patients with different genotypes, genetic basis may be different.

Key words： Han nationality Tibetan nationality Gastric cancer HIF-2&alpha gene Single nucleotide polymorphism

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	MA Jinhua1 MA Jinlan2 WANG Lijuan1 LI Jinzhang1 SHEN Cunfang1 MA Jinxiang3

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MA Jinhua1 MA Jinlan2 WANG Lijuan1 LI Jinzhang1 SHEN Cunfang1 MA Jinxiang3. The relationship between single nucleotide polymorphism of HIF-2α gene and susceptibility to gastric cancer of Qinghai area Tibetan, Han nationality[J]. 中国医药导报, 2019, 16(12): 9-12,22.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I12/9

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