多发性骨髓瘤患者骨髓组织中miRNA-181a、miRNA-373的表达及相关性分析

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Abstract Objective To investigate the expression of microRNA-181a (miRNA-181a) and miRNA-373 in bone marrow of patients with multiple myeloma (MM) and to explore its clinical significance. Methods A total of 67 patients with MM who were treated in Taizhou First People′s Hospital from March 2015 to March 2017 were selected as the case group, 40 patients with other blood diseases in the same period were selected as the case control group and 40 healthy people were selected as the healthy control group. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of miRNA-181a and miRNA-373 in bone marrow tissues of each group. The differences in the expression of miRNA-181a and miRNA-373 between these groups and their correlation with clinicopathological features of MM were analysed. Pearson linear correlation analysis were used to show the correlation between miRNA-181a and miRNA-373 expression. Results Compared with the case control group and the healthy control group, the expression of miRNA-181a was significantly higher in the case group and the expression of miRNA-373 was significantly lower (P < 0.05), but there was no difference in the expression of miRNA-181a and miRNA-373 between the case control group and the healthy control group (P > 0.05). The expression of miRNA-181a and miRNA-373 in bone marrow tissue of MM patients was related to tumor stage and tumor differentiation (P < 0.05), but not related to age, gender, cytological type and lymph node metastasis (P > 0.05). There was a significant negative correlation between miRNA-181a and miRNA-373 expression in the bone marrow of the case group (r = -0.696, P ＜ 0.05). There was no significant correlation between the case control group and the healthy control group (r = 0.151, 0.179, P > 0.05). Conclusion The expression of miRNA-181a is increased in MM bone marrow tissue, and the expression of miRNA-373 is decreased. Both of them participate in the tumor development and progression, which may become a new marker for diagnosis, treatment and prognosis of MM.

Key words： Multiple myeloma MicroRNA-181a MicroRNA-373 Correlation

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	WANG Xianting1 ZHENG Liangda1 XIE Jing2 JIANG Wenhua1

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WANG Xianting1 ZHENG Liangda1 XIE Jing2 JIANG Wenhua1. Expression and correlation analysis of miRNA-181a and miRNA-373 in bone marrow tissue of patients with multiple myeloma[J]. 中国医药导报, 2019, 16(11): 85-88,104.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I11/85

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