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| Ultrasonic extraction and antioxidant activity of total polyphenols from Myrtle root |
| YIN Huihui1 LIU Wei1 ZHAO Wu1 YANG Wuning2 MENG Fei1 JIANG Yuanming1 QIN Zhenhua1 SUN Jianhua1 |
| 1.Guangxi Veterinary Research Institute Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Zhuang Autonomous Region, Nanning 530001, China; 2.Guangxi Veterinary Drug Control Institute, Guangxi Zhuang Autonomous Region, Nanning 530001, China |
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Abstract Objective To study the ultrasonic extraction technology and antioxidant activity of total polyphenols from the root of Myrtle. Methods The extraction process of total polyphenols from the root of Myrtle was optimized by single factor and orthogonal experiment, and the absorbance of total polyphenols from the root of Myrtle was determined by Foline reagent under ultraviolet-visible spectrophotometer, and the extraction yield was calculated. The antioxidant activity was evaluated by 1,1-Diphyeny1-2-picrylhydrazyl free radical (DPPH·) scavenging rate and total antioxidant activity test. Results The optimum extraction conditions were ethanol concentration 60%, solid-liquid ratio 1∶40 (g/mL), extraction temperature 60℃, extraction time 50 min. Under these conditions, the extraction yield was 78.03 mg/g. The total antioxidant activity and DPPH· scavenging rate of total polyphenols in the root of Myrtle were higher than that of ascorbic acid. Conclusion Ultrasound extraction is suitable for the extraction of total polyphenols from the root of Myrtle, which is a natural antioxidant and free radical scavenger.
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