脊髓背角P物质、ERK1/CREB信号通路激活在大鼠椎间盘源性内脏痛形成机制中的作用

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Abstract Objective To investigate the molecular mechanisms of discogenic visceral pain resulted from autologous degenerative nucleus pulposus (NP) injection. Methods A total of 24 male SD rats were divided into na?觙ve group (n = 8), sham group (n = 8) and NP-treated group (n = 8) according to random number table method, with 8 rats in each group. Under fluoroscopic, autologous degenerative NP suspension was injected into right sympathetic trunk of rats in NP-treated group and the same dose of saline was administrated to right sympathetic trunk of animals in sham group, while the rats in the na?觙ve group did not receive any treatment. After 14 days postoperatively, the expression of substance P (SP), extracellular regulated protein kinases 1/2 (ERK1/2), p-ERK1/2, cyclic AMP response element binding protein (CREB) and p-CREB in the L1-L3 spinal cord of each group was analyzed by Western blot. Results The SP, p-ERK1, p-CREB expression in NP-treated group was significantly increased compared with those in na?觙ve group and control group (P ＜ 0.05), while there was no significant difference in p-ERK2 expression among the three groups (P ＞ 0.05). Conclusion The development of discogenic visceral pain induced by autologous degenerative NP injection is concerned with SP release and p-ERK1/CREB signaling pathway activation.

Key words： Spinal dorsal horn Visceral pain Intervertebral disc Rat Signaling pathway

Fund:北京市医院管理局青年人才培养“青苗”计划项目（QML20160807）；
首都医科大学基础-临床科研合作基金课题（16JL43）。

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	TANG Yuanzhang SUN Chenli GUO Yu′na YANG Liqiang WU Baishan WEI Ya NI Jiaxiang▲

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TANG Yuanzhang SUN Chenli GUO Yu′na YANG Liqiang WU Baishan WEI Ya NI Jiaxiang▲. Effect of substance P and ERK1/CREB signaling pathway activation in spinal dorsal horn on the mechanism of discogenic visceral pain development in rats[J]. 中国医药导报, 2019, 16(10): 7-10.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I10/7

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