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| Effect of Paclitaxel on autophagy of human nasopharyngeal carcinoma cells in vitro |
| SHI Xiaojing ZHENG Mingfen WAN Renqiang ZHANG Shenhua |
| Department of ORL-HNS, Guangdong Provincial Second General Hospital, Guangdong Province, Guangzhou 510000, China |
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Abstract Objective To investigate the effect of Paclitaxel on autophagy in human nasopharyngeal carcinoma cells in vitro. Methods Human nasopharyngeal carcinoma cell line CNE2 was cultured in vitro. The proliferation inhibition of Paclitaxel on human nasopharyngeal carcinoma cell line CEN2 was detected by MTT assay. The effect of Paclitaxel on CEN2 cell apoptosis was analyzed. The cell cycle changes and radiosensitization of Paclitaxel on CNE2 cell were observed and analyzed. Results After 12 h of treatment, the proportion of surviving cells was significantly lower than that of the control group (P < 0.05); after 48 h of treatment, the proportion of surviving cells was significantly lower than that of other groups (P < 0.05); after 24 h of treatment, the proportion of early apoptotic cells was the highest, significantly higher than that of other groups (P < 0.05); after 48 h of treatment, the proportion of cell necrosis and late apoptotic cells was the highest, significantly higher than that of other groups (P < 0.05). In the whole cell cycle, the proportion of G0/G1 cycle in blank control group was the highest, and significantly higher than that in other groups (P < 0.05); the proportion of G2/M cycle in paclitaxel group was the highest, and significantly higher than that in other groups (P < 0.05); there was no significant difference in the proportion of G0/G1, G2/M and S cycle in radiotherapy group (P > 0.05); and the proportion of G2/M cycle in paclitaxel + radiotherapy group was the highest (P <0.05). The apoptotic rate of paclitaxel + radiotherapy group was significantly higher than that of other groups (P < 0.05). Conclusion Paclitaxel has obvious toxic effects on human nasopharyngeal carcinoma CNE2 cells, which can inhibit the division of human nasopharyngeal carcinoma CNE2 cells, and can treat nasopharyngeal carcinoma to some extent. As the concentration of paclitaxel increases, its apoptosis effect on CNE2 cells is stronger. Paclitaxel combined with radiotherapy can further increase the apoptosis rate of CNE2 cells.
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