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| Expression and significance of IL-35 and IL-17 in intestinal mucosa of rats with ulcerative colitis |
| ZHAO Junya LI Wei LI Wei SUN Hongyu Li Xin GUAN Jie▲ |
| School of Medical Technology, Qiqihar Medical University, Heilongjiang Province, Qiqihar 161000, China |
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Abstract Objective To investigate the expression level and significance of interleukin-17 (IL-17) and IL-35 in intestinal mucosa of Ulcerative colitis UC rats. Methods Six-five healthy SD male rats were divided into control group (n = 12) and experimental group (n = 53). The blank control group was not treated, and 12 SD rats were randomly selected from the experimental group for the preliminary experiment, the most appropriate dose of TNBS (2, 4, 6-trinitrobenzenesulfonic acid) modeling agent was selected to build UC animal model. Changes in body weight and general conditions (mental status, stool shape, color, etc.) were observed daily and scored according to disease activity level (DAI) since it has been formally modeled. The rest in the control group were sacrificed on day 3, day 7 and day 14, respectively. The remaining rats were formally modeled and divided into group A , group B and group C with 12 rats in each group according to the degree of ulcer (5 rats died accidently). Colonic mucosa damage index (CMDI) was used to score the colon and determine the degree of ulcer. The contents of IL-17 and IL-35 in the intestinal mucosa were detected by ELISA method, and the pathological changes of the colon were evaluated at the same time. The correlation between the levels of IL-17 and IL-35 and the degree of ulcer was obtained. Results Comparison between the experimental group and the blank group, DAI score and CMDI score were increased, and the pathological changes of colon tissue in the experimental group were significant under the microscope (P ＜ 0.05). The level of IL-17 in the intestinal mucosa of rats in each group increased significantly with the aggravation of the ulcer, and the level of IL-35 decreased significantly, with statistically significant differences (P ＜ 0.05). Conclusion During the development of ulcerative colitis, cytokines play an important role as mediators of inflammation. The content of IL-17 is positively correlated with the inflammatory response and may promote the occurrence of inflammatory response, while the content of IL-35 is negatively correlated with the degree of inflammation, and may be involved in inhibiting the occurrence and development of inflammatory response.
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