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| The effect of chitosan oligosaccharide on the level of endoplasmic reticulum stress and inflammatory factors released in adipose tissue of obese mice induced by high-fat diet |
| YANG Shuo WANG Bin XIA Wenshui |
| Collaborative Innovation Center, School of Food Science and Engineering, Jiangnan University, Jiangsu Province, Wuxi 214122, China |
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Abstract Objective To investigate the effect of chitosan oligosaccharide on the level of endoplasmic reticulum stress and inflammatory factors released in adipose tissue of obese mice induced by high-fat diet. Methods Thirty C57BL/6J mice were divided into normal diet group (Control group), high-fat diet group (HF group) and high-fat diet+chitosan oligosaccharide group (HF+COS group) according to random number table method, with 10 mice in each group. After being fed for 16 weeks, glucose tolerance test and insulin tolerance test were carried out. Serum was collected to detect lipid profiles, mice body weights, perirenal and subcutaneous adipose tissue weights were estimated within groups. In addition, the protein and mRNA expression of endoplasmic reticulum stress markers glucose regulatory protein (GRP-78) and endoplasmic reticulum kinase (PERK) from adipose tissue were assessed. Meanwhile, the changes of nuclear factor-κB (NF-κB) inflammatory pathways in adipose tissue were also detected. Results Compared with the control group, the levels of glucose, lipid, the body weights and the size and weight of perirenal and subcutaneous adipocytes in HF group were significantly increased (P ＜ 0.05 or P < 0.01), the mRNA and protein expression of GRP-78 and PERK were increased significantly (P ＜ 0.05). And the activation states of NF-κB pathway was measured accordingly. Compared with HF group, except for total cholesterol, the mentioned indicators in HF+COS group were significantly decreased (P ＜ 0.05). Conclusion Chitosan oligosaccharide can lower blood lipids, reduce the excessive accumulation of adipose tissue and relieve endoplasmic reticulum stress in mice fed on high-fat diet. Chitosan oligosaccharide inhibits the activation of NF-κB, resulting in the reduced release of inflammatory factors which is likely to contribute to the insulin resistance alleviation.
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