不同冻存时间对人颗粒脂肪活性影响的实验研究

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Abstract Objective To explore the effect of different cryopreservation time on human adipose cell vitality, and seek a more satisfactory granular fat cryopreservation time, to provide a theoretical reference for autologous fat transplantation in clinic. Methods The fat granule samples from interior thigh of 41 healthy women (20-40 years old) that underwent conventional liposuction were collected in Guangmei Medical Plastic and Cosmetic Outpatient Department from June to October 2016. All fat granule samples were cryopreserved in liquid nitrogen freezer of -196℃ after washed twice by physiological saline and placed quiescently to isolate them from the physiological saline and then an equal volume of freezing protection liquid was added into the fat granule samples. The samples that had been stored for 3 months (group A) and 6 months (group B) were selected to test the activity of frozen fat after thawed. The morphology and the death cell percentage of cryopreserved granule fat cells was observed and calculated respectively by hematoxylin-eosin (HE) staining and trypan-blue staining. The creatine kinase level was tested to calculate fat cell enzyme vitality. The CD105 on adipose derived stem cells (ADSCs) was detected by immunohistochemical methods to compare the effect of different cryopreservation time on the fat cell activity in vitro. Results Fat cell count analysis on the survival rate of fat cells and the determination of creatine kinase showed no statistically significant differences in the fat cell survival rates between group A and group B under the same cryopreservation temperature (P ＞ 0.05). The comparative observation of the fat cell morphology by HE staining under the microscope showed that all cell membranes keep well in the two groups. Immunohistochemical staining of CD105 showed that there were only a few of ADSCs in group A. Conclusion Cryopreservation of human granular adipose tissue with liquid nitrogen is safety and effective. The fat cells can be cryopreserved at -196℃ for 6 months or even longer time, and this cryopreserved temperature is ideal.

Key words： Fat granule Cryopreservation Stored time Fat cell vitality

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	WEI Guoqian1* GU Luosha2* WU Mingzheng3 BAI Jie1 GU Jinsong1▲ YU Jiang1 YAN Runhu1 ZHANG Yi1

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WEI Guoqian1* GU Luosha2* WU Mingzheng3 BAI Jie1 GU Jinsong1▲ YU Jiang1 YAN Runhu1 ZHANG Yi1. Experimental study on the effect of different cryopreservation time on human fat granule[J]. 中国医药导报, 2019, 16(6): 16-19.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I6/16

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