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| Analysis of S region gene mutation in patients with HBsAg+/HBsAb+ chronic hepatitis B |
| SUN Yu1* ZHANG Honghai1* CHEN Dexi2 WEI Feili2 GUO Xianghua2 SHI Ying2 |
1.Minimally Invasive Interventional Center of Oncology, Beijing You′an Hospital, Capital Medical University, Beijing 100069, China;
2.Beijing Institute of Liver Diseases, Beijing 100069, China |
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Abstract Objective To study the S region gene mutation in patients with hepatitis B surface antigen (HBsAg) +/ hepatitis B surface antibody (HBsAb) + chronic hepatitis B (CHB). Methods From June 2012 to October 2017, 32 cases of CHB patients with double positive HBsAg and HBsAb in Beijing You′an Hospital, Capital Medical University (hereinafter referred to as “our hospital”) were selected as subjects (HBsAg+/HBsAb+ group),79 cases of CHB patients diagnosed with HBsAg+ and HBsAb- in our hospital in the same period were randomly selected as the control group (HBsAg+/HBsAb- group). The plasma viral DNA was isolated, the S region gene was amplified by PCR, and the mutation was analyzed by sequencing. Results All patients in the HBsAg+/HBsAb+ group had S-region mutation (100%), and the frequency was significantly higher than that in the HBsAg+/HBsAb- group (84.81%) (P ＜ 0.05). Moreover, the mutations were mostly concentrated in the subunits 1 and 3 of S region. The proportion of multiple site mutations in S region in HBsAg+/HBsAb+ group was significantly higher than that in HBsAg+/HBsAb- group (P ＜ 0.01). Conclusion The increased frequency of S region gene mutation and the presence of multiple mutations are closely related to the change of S antigen antibody in CHB patients, and the accumulation of this mutation indicates an adverse disease process.
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