胃癌组织中FHIT、Bcl-2蛋白表达与幽门螺杆菌感染的关系

Abstract
Figure/Table
References (23)
Related Citation (15)

Download: PDF (596 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the relationship between fragile histidine triad (FHIT) and Bcl-2 protein expression in gastric cancer tissues and helicobacter pylori (Hp) infection. Methods From January 2013 to January 2017, 80 gastric carcinoma tissue samples were collected after radical gastrectomy in the 464th Hospital of PLA, and 43 paracancerous tissues were selected. Rapid urease assay paper and Warthin-Starry staining were used to detect whether the tissue samples were infected with Hp, and the expressions of FHIT and Bcl-2 in the tissue samples were detected by immunohistochemical staining. Results FHIT positive expression rate in gastric carcinoma tissues was lower than that in para-carcinoma tissues (P < 0.05), Bcl-2 positive expression rate in gastric carcinoma tissues was higher than that in para-carcinoma tissues (P < 0.05). The expression of FHIT in gastric carcinoma tissues was related to lymphatic metastasis, distant metastasis, degree of differentiation, degree of infiltration and Hp infection (all P < 0.05). Bcl-2 expression was related to tumor diameter, differentiation degree, infiltration degree and Hp infection (all P < 0.05). The positive rate of FHIT was negatively correlated with Hp infection (r = -0.295, P < 0.05), and the positive rate of Bcl-2 was positively correlated with Hp infection (r = 0.393, P < 0.05). Conclusion The expression of FHIT protein in gastric carcinoma tissues is weakened, and the expression of Bcl-2 protein is enhanced, which are closely related to Hp infection, and the three interactions lead to the occurrence of gastric carcinoma.

Key words： Gastric carcinoma Helicobacter pylori Fragile histidine triad Bcl-2

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHAO Kaifeng1 LIU Huiying2 JIANG Daoliang1 MIAO Ye2 ZHANG Jing1

Cite this article:

ZHAO Kaifeng1 LIU Huiying2 JIANG Daoliang1 MIAO Ye2 ZHANG Jing1. Relationship between FHIT and Bcl-2 protein expression and Helicobacter pylori infection in gastric carcinoma[J]. 中国医药导报, 2019, 16(5): 100-103.

URL:

http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I5/100

[1] 万军，张子其，朱成王，等.胃镜普查及随访对老年人胃癌的诊断价值[J].中华老年医学杂志，2001，20（2）：103-104.
[2] 徐飚，王建明.胃癌流行病学研究[J].中华肿瘤防治杂志，2006，13（1）：1-7.
[3] 秦叔逵，龚新雷.晚期胃癌化疗的现状和新进展[J].临床肿瘤学杂志，2006，11（9）：641-652.
[4] Ohta M，Inoue H，Cotticelli MG，et al. The FHIT gene，spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t（3；8）breakpoint，is abnormal in digestive tract cancers [J]. Cell，1996，84（4）：587-597.
[5] 杨健，胡宏波，贾安平，等.FHIT基因在胃癌中的甲基化表达及其与胃癌临床病理特征的关系[J].海南医学，2011， 22（10）：41-43.
[6] 刘改芳，林三仁.幽门螺杆菌感染与胃癌关系的研究进展[J].中国实用内科杂志，2003，23（9）：573-574.
[7] 杨勇，田敏.胃镜活检病理对胃癌诊断价值分析[J].现代消化及介入诊疗，2017，22（5）：653-654.
[8] 王霞.内镜活检胃癌组织中HER-2蛋白表达及临床意义[J].现代消化及介入诊疗，2017，22（3）：307-310.
[9] 孙燕，刘南植.幽门螺杆菌致胃上皮细胞凋亡的机制及其在胃癌发生中的作用[J].国际内科学杂志，2004，31（10）：419-421.
[10] 苑博，李建功，成雨.螺旋杆菌与胆管癌的关系[J].临床肝胆病杂志，2018，34（2）：444-447.
[11] Watanabe T，Tada M，Nagai H，et al. Helicobacter pylori infection induces gastric cancer in mongolian gerbils [J]. Gastroenterology，1998，115（3）：642-648.
[12] Hu B，Wang H，Wang X，et al. Fhit and CHK1 have opposing effects on homologous recombination repair [J]. Cancer Res，2005，65（19）：8613.
[13] Pekarsky Y，Zanesi N，Palamarchuk A，et al. FHIT：from gene discovery to cancer treatment and prevention [J]. Lancet Oncol，2002，3（12）：748-754.
[14] Barnes LD，Garrison PN，Siprashvili Z，et al. Fhit，a putative tumor suppressor in humans，is a dinucleoside 5′，5"′-P1，P3-triphosphate hydrolase [J]. Biochemistry，1996，35（36）：11529-11535.
[15] Baffa R，Veronese ML，Santoro R，et al. Loss of FHIT expression in gastric carcinoma [J]. Cancer Res，1998，58（20）：4708.
[16] Huiping C，Kristjansdottir S，Bergthorsson JT，et al. High frequency of LOH，MSI and abnormal expression of FHIT in gastric cancer [J]. Eur J Cancer，2002，38（5）：728-735.
[17] Rocco A，Schandl LJ，Wang H，et al. Loss of FHIT protein expression correlates with disease progression and poor differentiation in gastric cancer [J]. J Cancer Res Clin Oncol，2003，129（2）：84-88.
[18] Zhang H，Fang DC，Wang RQ，et al. Effect of Helicobacter pylori infection on expression of Bcl-2 family members in gastric adenocarcinoma [J]. World J Gastroenterol，2004，10（2）：227-230.
[19] Lam M，Dubyak G，Chen L，et al. Evidence that BCL-2 represses apoptosis by regulating endoplasmic reticulum-associated Ca2+fluxes [J]. Proc Natl Acad Sci U S A，1994，91（14）：6569-6573.
[20] Hockenbery DM，Oltvai ZN，Yin XM，et al. Bcl-2 functions in an antioxidant pathway to prevent apoptosis [J]. Cell，1993，75（2）：241-251.
[21] Korsmeyer SJ. BCL-2 gene family and the regulation of programmed cell death [J]. Cancer Res，1994，59（3）：387.
[22] 刘海峰，刘为纹，房殿春，等.bcl-2蛋白在胃癌组织中的表达和意义[J].肿瘤防治研究，1997，24（5）：269-271.
[23] Jr BW，Martini MR，Squassoni AC，et al. Effects of Helicobacter pylori infection on the expressions of Bax and Bcl-2 in patients with chronic gastritis and gastric cancer [J]. Dig Dis Sci，2010，55（1）：111-116.