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| Effects of ginseng on intestinal mucosa and the levels of serum CRP, TNF-α and IL-6 in mice after infection |
| FENG Qilin1 DENG Aiping1▲ LIU Jue1 FAN Yanbo2 |
1.Department of Pharmacy, Wuhan Central Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei Province, Wuhan 430014, China;
2.Department of Scientific Research, Wuhan Hospital of Traditional Chinese Medicine, Hubei Province, Wuhan 430014, China |
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Abstract Objective To investigate the effects of ginseng on intestinal mucosa and the levels of serum C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in mice after infection. Methods Among 60 mice, 15 mice were randomly selected as blank control group, and the remaining 45 mice were used to prepare severe abdominal infection model (infected group). Then 45 mice models were divided into three groups by the random number table method: model group, anti-inflammatory group, anti-inflammatory + ginseng group, with 15 mice in each group. The anti-inflammatory group was given Imipenem/Cilastatin 333.33 mg/kg (intraperitoneal injection, once 12 h); the anti-inflammatory + ginseng group was given intragastric administration of ginsenoside Rb1 (purity＞98%) 50 mg/(kg·d) on the basis of the anti-inflammatory group; the model group was given the same amount of sterile saline by intraperitoneal injection. Blood samples were taken at 24, 48 h and 72 h after treatment. Five rats in each group were used to observe the changes of CRP, TNF-α and IL-6 levels in the intestinal mucosa of mice in each group; the terminal ileum was harvested and HE staining was performed. The intestinal mucosal layer was observed and the intestinal epithelial cell apoptosis index was calculated. Results After severe intraperitoneal infection, mice intestinal mucosal atrophied, intestinal epithelial cell apoptosis increased significantly, and the more obvious the intestinal mucosal atrophied, the greater the number of intestinal mucosal epithelial cell apoptosis was. After treatment for 72 h, the levels of CRP, TNF-α and IL-6 in mice with severe intraperitoneal infection of anti-inflammatory + ginseng group were significantly decreased (P ＜ 0.05 or P ＜ 0.01), and the decrease range was greater than that of anti-inflammatory group (P ＜ 0.05); the apoptotic index of anti-inflammatory + ginseng group was significantly lower than that of the model group and the anti-inflammatory group (P < 0.05 or P < 0.01). Conclusion Severe abdominal infection can cause intestinal mucosal atrophy, intestinal mucosal atrophy is related to the number of intestinal epithelial cell apoptosis; ginseng has a protective effect on the intestinal mucosa, which can improve the symptoms of intestinal mucosal atrophy, reduce the impact on intestinal mucosal secretions.
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