利用bFGF提高直接重编程诱导成纤维细胞向心肌细胞转化效率的实验研究

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Abstract Objective To investigate the effects of basic fibroblast growth factor (bFGF) on the transformation of direct reprogramming-induced transdifferentiation of fibroblasts into cardiomyocytes. Methods Direct reprogramming was performed to induce rat tail fibroblasts into cardiomyocytes by defined factors GATA-4, Mef2c and Tbx5. Then the cells were divided into two groups, one group was cultured normally, and the other group was cultured with 50 μg/L bFGF. Two weeks later, the differentiation of cardiomyocytes was identified by detection of the expression of cardiac specific protein such as cardiac troponin T (cTNT), α-myosin heavy chain (α-MHC), GATA binding protein 4 antibody (GATA-4) and connexin 43 (CX43) and analysis of electrophysiology. And the differentiation rates of the two groups were compared. Results Western blot and immunofluorescence test showed that the expression of cTNT, α-MHC, GATA-4 and CX43 significantly up-regulated after exposure to bFGF (P ＜ 0.01). And the further analysis of electrophysiology revealed that the electrophysiological characteristics of these cells were very similar to those of cardiomyocytes, which was identified as cardiac differentiation. Conclusion Fibroblasts can be induced by direct reprogramming to differentiate into cardiomyocytes, which can be promoted by bFGF. This study may provide a new method to harvest cardiomyocytes.

Key words： Direct reprogramming Cardiomyocytes Basic fibroblast growth factor

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	REN Jie ZHAO Long▲ SONG Zhenxiang LI Wei

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REN Jie ZHAO Long▲ SONG Zhenxiang LI Wei. Experimental study on the efficiency of bFGF promoting direct reprogramming-induced transdifferentiation of fibroblasts into cardiomyocytes[J]. 中国医药导报, 2019, 16(2): 17-19,28.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I2/17

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