表皮生长因子协同体预处理对大鼠脑缺血再灌注的保护作用

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Abstract Objective To study the effect of epidermal growth factor-coordination (EGF-c) on cerebral ischemia reperfusion (IR) in rats as well as its mechanism. Methods Thirty male SD rats were divided into sham group, ischemia reperfusion group (IR group), EGF-c pretreatment and IR group (EGF-c group) by random number table method. The middle cerebral artery occlusion (MCAO) model of rats was made by suture method. 24 hours after operation, the cerebral infarction volume was detected by 2% triphenyl tetrazolium chloride (TTC) staining, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in brain tissue were measured by enzyme-linked immunosorbent assay (ELISA), the expression of high mobility group protein 1 (HMGB1) was examined by Western blot. Results Compared with sham group, the infarction volume was increased (P ＜ 0.01), the SOD activity was decreased (P ＜ 0.01), the contents of MDA and HMGB1 were increased in IR group (P ＜ 0.01). Compared with IR group, the infarction volume was decreased (P ＜ 0.05), SOD activity was increased (P ＜ 0.01), the content of MDA was decreased (P ＜ 0.01), the expression of HMGB1 was decreased in EGF-c group (P ＜ 0.05). Conclusion EGF-c pretreatment may play a protective effect on IR of rats by improving the SOD activity, decreasing the content of MDA and the expression of HMGB1.

Key words： Epidermal growth factor-coordination Cerebral ischemia reperfusion Superoxide dismutase Malondialdehyde High mobility group protein 1

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	QU Jing XU Na AO Ting ZHANG Jun ZHANG Qin XIAO Shuying ZHANG Ruihua▲

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QU Jing XU Na AO Ting ZHANG Jun ZHANG Qin XIAO Shuying ZHANG Ruihua▲. Protective effect of epidermal growth factor-coordination pretreatment on cerebral ischemia reperfusion in rats[J]. 中国医药导报, 2019, 16(2): 8-11,16.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I2/8

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