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| Effect of Ningdong Granules on metabolism of dopaminergic neurons |
| LI Mengying1 JIANG Liyuan1 SUN Kexing2 Xiaohong LI2 LI Jijun2▲ |
1.Quzhou College of Technology, Zhejiang Province, Quzhou 324000, China;
2.Department of Integrated Traditional Chinese and Western Medicine, Shanghai Children′s Medical Center, Shanghai Jiaotong University, Shanghai 200127, China |
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Abstract Objective To study the effect of Ningdong Granules on the metabolism of dopaminergic (DA) neurons. Methods Twelve 18-days-old fetuses were randomly divided into Ningdong Granules (NDG) group, control (CTR) group, haloperidol (Hal) group and normal (Normal) group, with 3 rats in each group. DA neurons of each group were separately isolated and cultured, and tyrosine hydroxylase (TH) anti-staining was performed on DA neuron cells by immunofluorescence indirect method. Neuron axon anti-Tau1 anti-labeling combination with FITC fluorescence (green light) Ⅱ anti-labeling, neuron axon anti-Tau1 labeling combined with Cy 3 fluorescence (red light) Ⅱ anti-labeling, DA neuron cells and neuron axons were identified by fluorescent staining. The contents of MAO, DA, 5-HT, HVA and 5-HIAA in DA neuron cell culture medium of 18-days-old fetuses before and 1, 6, 12 and 24 h after drug intervention were detected by ELISA, and the metabolism of metacells of DA energetic spirit in each group was recorded. Results Compared with 0 h, the contents of MAO, DA, 5-HT and HVA in the cell culture medium of NDG group increased significantly after 6 and 12 h of intervention (P < 0.05); compared with CTR group at the same time, there was no significant difference in the contents of MAO, DA, 5-HT and HVA in the cell culture medium of Hal group after 0-24 h of intervention (P > 0.05); after 0-24 h of intervention, the 5-HIAA content in cell culture of fetuses in each group had no significant difference (P > 0.05). Conclusion NDG can promote the metabolism of DA neurons, but has no significant effect on the metabolism of 5-HT.
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[1] Willcutt EG. The prevalence of DSM-IV attention-deficit/hyperactivity disorder：a meta-analytic review [J]. Neurotherapeutics，2012，9（3）：490-499.
[2] 汤海霞，李安源，李继君，等.宁动颗粒对抽动-秽语综合征患儿IL-12、TNF-α的影响[J].中国中西医结合杂志，2014，34（4）：435-438.
[3] 杨圣海，王立文，王珺，等.健脑止抽颗粒联合硫必利治疗儿童抽动秽语综合征的效果[J].中国医药导报，2018， 15（17）：65-67，71.
[4] 律萌萌，王雪峰，张秀英.菖麻熄风片治疗抽动症共患多动症（肝风挟痰证）临床研究[J].亚太传统医药，2018，14（4）：186-188.
[5] 陈自佳，于文静，王素梅.脾虚证多发性抽动症小鼠模型的建立及评价[J].环球中医药，2016，9（9）：1055-1058.
[6] 李福轮，谢晴牧，赵乾龙，等.中国儿童注意缺陷多动障碍患病率的Meta分析[J].临床荟萃，2017，32（12）：1079-1083.
[7] 陈果，向娟，邓鸿博，等.中药治疗小儿抽动秽语综合征的系统评价[J].中医药导报，2017，23（7）：87-89，99.
[8] Jijun L，Zaiwang L，Anyuan L，et al. Abnormal expression of dopamine and serotonin transporters associated with the pathophysiologic mechanism of Tourette syndrome [J]. Neurol India，2010，58（4）：523-529.
[9] 杨德爽，孟州令，国文文，等.柴胡加龙骨牡蛎汤对多发性抽动症模型大鼠行为学和多巴胺系统的影响[J].中国中西医结合杂志，2018，38（1）：76-79.
[10] 张璠，郑宏，马丙祥，等.六味地黄丸对多发性抽动症模型大鼠行为及脑多巴胺、高香草酸水平的影响[J].中医学报，2017，32（6）：981-984.
[11] 高汉媛，史正刚，李小芹，等.IDPN诱导TS模型大鼠纹状体DA系统的变化及菖蒲郁金汤的干预作用[J].时珍国医国药，2016，27（6）：1367-1369.
[12] 张峰.天麻素与宁动颗粒对Tourette综合征大鼠纹状体多巴胺双向调节作用的研究[D].济南：山东大学，2015.
[13] 孙继超，韩新民，郭玉清，等.270例儿童多动症中医证型分布及演变规律探讨[J].辽宁中医杂志，2016，43（5）：908-910.
[14] 李继君，汤海霞，尹文静，等.宁动颗粒对不同中医证型抽动-秽语综合症模型大鼠刻板行为的影响[J].中国中西医结合杂志，2013，33（8）：1112-1115.
[15] 尹文静，牛笛，李继君.宁动颗粒对儿童抽动-秽语综合征伴睡眠障碍的影响[J].医药导报，2017，36（4）：413-416.
[16] 祖娜，陈光耀，方锦颖，等.多发性抽动症中医药动物实验研究现状及评价[J].医学综述，2017，23（17）：3460-3463，3468.
[17] 高超，吴永利，刘君婷，等.氟哌啶醇联合可乐定透皮贴剂治疗儿童难治性Tourette综合征及对Th1/Th2的影响[J].疑难病杂志，2016，15（12）：1259-1262.
[18] 方锦颖，陈琪，国文文，等.龟板对多发性抽动症模型大鼠的干预作用与对DA及D2受体的影响[J].环球中医药，2016，9（7）：781-784.
[19] Hauseux PA，Cyprien F，Cif L. Long-term follow-up of pallidal deep brain stimulation in teenagers with refractory Tourette syndrome and comorbid psychiatric disorders：about three cases [J]. Eur J Paediatr Neurol.2017， 21（1）：214-217.
[20] Sharma A，Couture J. A review of the pathophysiology，etiology，and treatment of attention-deficit hyperactivity disorder （ADHD） [J]. Ann Pharmacother，2014，48（2）：209-225.
[21] 方琼杰，王俊宏，姜思竹.从“心肝有余，脾肾不足”及“五神藏”理论辨治儿童多动症[J].中医杂志，2016，57（3）：254-256.
[22] 李燕，王新征，康珈宁，等.熄风静宁颗粒对Tourette综合征模型大鼠行为活动及脑纹状体多巴胺递质的影响[J].中国中医急症，2016，25（9）：1660-1662，1666.
[23] 梁友芳，农清清，覃健敏，等.儿童注意缺陷多动障碍与5-羟色胺R2A基因多态性的相关性研究[J].中国全科医学，2016，19（11）：1287-1291.
[24] 李继君.DAT及SERT在Tourette Syndrom模型大鼠中发病机制研究及TS的中医证候学调查[D].济南：山东大学，2011. |
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