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| Mitochondrial protective mechanism of Danqu Capsules against myocardial ischemia / reperfusion injury |
| TIAN Xin1 XU Pan2 LIU Chaofeng1 |
1.Department of Cardiology, Traditional Chinese Medical Hospital of Shaanxi Province, Shaanxi Province, Xi′an 710003, China;
2.Department of Clinical Medicine, Hubei University of Medicine, Hubei Province, Shiyan 442000, China |
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Abstract Objective To explore the mitochondrial protective mechanism of Danqu Capsules on myocardial ischemia /reperfusion injury. Methods Thirty healthy male SD rats were divided into 5 groups by random number table method (n = 6): sham operation group, model group, 300 mg/kg of Danqu Capsules group, 600 mg/kg of Danqu Capsules group, and Isosorbide Mononitrate group (10 mg/kg). The method of ligation of the left anterior descending branch of coronary artery was used to make model, and the changes of ST segment at different time points of ischemia / reperfusion were recorded. The levels of myocardial injury markers [cardiac troponin T (cTnT), creatine kinase isoenzyme (CK-MB)] were measured by enzyme-linked immunosorbent assay (ELISA) method. The ultrastructure of myocardial mitochondria was observed with transmission electron microscope, and the expression of mitochondrial fission protein p-Drp1 and fussion protein Mfn2 was measured by immunoblotting. Results 300, 600 mg/kg of Danqu Capsules groups could reduce the levels of myocardial injury markers (P ＜ 0.01), relieve the ST segment elevating of ECG (P ＜ 0.01), reduce mitochondrial fission of myocardial cells and lessen the expression of mitochondrial protein p-Drp1 (P ＜ 0.01), but there was no significant difference in the expression of mitochondrial fusion protein Mfn2 (P ＞ 0.05). Conclusion Danqu Capsules can alleviate myocardial ischemia / reperfusion injury, improve myocardial ischemia and reduce mitochondrial fission of myocardial cells, which may be related to the inhibition of the expression of mitochondrial fission protein p-Drp1.
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