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| Protective mechanism of retinoic acid on primary cultured alveolar type Ⅱ epithelial cells under hyperoxia |
| PAN Qiqi* MA Jinshuai* LIU Xiuxiang LYU Meng |
| Department of Neonatology, Affiliated of Binzhou Medical University, Shandong Province, Binzhou 256600，China |
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Abstract Objective To investigate protective mechanism of retinoic acid on primary cultured alveolar type Ⅱ epithelial cells under hyperoxia. Methods Alveolar type Ⅱ epithelial cells were isolated and purified from fetal rat lung tissue at 19 days of gestation. When the cells grew to the fused state (80% bottle bottom), the cells were divided into air group, hyperoxia group and hyperoxygenation + RA group. The apoptosis of AECⅡ was detected by flow cytometry, the β-catenin protein expression of Wnt signal pathway was detected by Western blot, and the β-catenin gene expression was detected by PCR. Results Compared with the air group and the hyperoxygenation + RA group, the AECⅡ number of AnnexinV (+) PI (-) and AnnexinV (+) PI (+) markers in the hyperoxia group was significantly higher at 24 h of exposure (P < 0.01), and the cell number was significantly reduced after RA addition. Compared with the air group, the expression of β-catenin protein of the hyperoxia group was significantly decreased, the difference was statistically significant (P < 0.01), while compared with the high oxygen group, the expression of β-catenin protein of the hyperoxygenation + RA group was upregulated significantly (P < 0.01). Conclusion Hyperaerobic environment can lead to massive apoptosis and necrosis of AECⅡ, and RA can reduce apoptosis of this cell by up-regulating the expression of β-catenin protein, thus playing a protective role in hyperoxic lung injury.
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