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| Effect of naringenin pretreatment on the energy metabolism after heart injury induced by ischemia/reperfusion in rats |
| MENG Limin |
| The Second Department of Internal Medicine, Handan Branch, Jizhong Energy Fengfeng Group Hospital, Hebei Province, Handan 056200, China |
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Abstract Objective To investigate the effect of naringenin pretreatment on the energy metabolism after heart injury induced by ischemia/reperfusion in rats. Methods According to random number table method, 50 adult male Sprague-Dawley (SD) rats were divided into five groups (n = 10): blank control group, modle group, and low, medium, high dose of naringenin group (5, 10, 20 μmol/L). The heart of rat in each group was treated with 30 min global ischemia followed by 120 min reperfusion; the rats of low, medium, high dose of naringenin group were treated with 5, 10, 20 μmol/L of naringenin perfusion before ischemia; after reperfusion, the myocardial content of lactate dehydrogenates and myocardial infarct size in each group were examined, the activities of Na+/K+-ATPase and Ca2+/Mg2+-ATPase in myocardial tissue were detected. Results After ischemia/reperfusion injury, compared with modle group, the contents of lactate dehydrogenates in low, medium, high dose of naringenin group were significantly decreased (P ＜ 0.05 or P ＜ 0.01), the myocardial infarct size was significantly reduced (P ＜ 0.05 or P ＜ 0.01), the activities of Na+/K+-ATPase and Ca2+/Mg2+-ATPase in myocardial tissue were significantly improved (P ＜ 0.05). Conclusion Narigenin palys a cardioprotective role may be through enhancing the myocardial energy metabolism of rats with ischemia/reperfusion.
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[1] Tsai SJ，Huang CS，Mong MC，et al. Anti-inflammatory and antifibrotic effects of naringenin in diabetic mice [J]. J Agric Food Chem，2012，60（1）：514-521.
[2] Lou C，Zhang F，Yang M，et al. Naringenin decreases invasiveness and metastasis by inhibiting TGF-beta-induced epithelial to mesenchymal transition in pancreatic cancer cells [J]. PLoS One，2013，7（12）：e50956.
[3] Karuppagounder V，Arumugam S，Thandavarayan RA，et al. Naringenin ameliorates daunorubicin induced nephrotoxicity by mitigating AT1R ERK1/2-NFκB p65 mediated inflammation [J]. Int Immunopharmacol，2015，28（1）：154-159.
[4] Rahigude，A，Bhutada，P，Kaulaskar，S，et al. Participation of antioxidant and cholinergic system in protective effect of naringenin against type-2 diabetes-induced memory dysfunction in rats [J]. Neuroscience，2012，9（26）：62-72.
[5] Tsai SJ，Huang CS，Mong MC，et al. Anti-inflammatory and antifibrotic effects of naringenin in diabetic mice [J]. Agric Food Chem，2012，60（1）：514-521.
[6] Yoshida H，Watanabe W，Oomagari H，et al. Citrus flavonoid naringenin inhibits TLR2 expression in adipocytes [J]. Nutr Biochem，2013，24（7）：1276-1284.
[7] Ramprasath T，Senthamizharasi M，Vasudevan V，et al. Naringenin confers protection against oxidative stress through Upregulation of Nrf2 target genes in cardiomyoblastcells [J]. J Physiol Biochem，2014，70（2）：407-415.
[8] Jennings RB，Sommers HM，Smyth GA，et al. Myocardial necrosis induced by temporary occlusion of a coronary artery in the dog [J]. Arch Pathol，1960，70：68-78.
[9] 孟利敏，吴绍华，席树强，等.柑橘素预处理对大鼠离体心脏缺血再灌注损伤的保护作用[J].中国医药导报，2017， 14（18）：20-24.
[10] Ghiasl R，Mohammad M，Majidina M，et al. The effects of mebudipine on myocardial arrhythmia induced by ischemia-reperfusion injury in isolated rat heart [J]. Cell Mol Biol（Noisyle-grand），2016，62（13）：15-20.
[11] Meng G，Wang J，Xiao Y，et al. GYY4137 protects against myocardial ischemia and reperfusion injury by attenuating oxidative stress and apoptosis in rats [J]. J Biomed Res，2015，29（3）：203-213.
[12] 刘丹，金良友，冯晓灵，等.柚皮苷对心肌缺血- 再灌注损伤大鼠血流动力学的影响[J].重庆医学，2018，47（6）：733-739.
[13] 胡珍，陈景瑞，魏静，等.冠状动脉结扎制备大鼠心肌梗死模型及评价实验研究[J].天津中医药，2016，33（2）：90.
[14] 国科，刘达兴，容松.心肌缺血再灌注损伤的发生机制及其防治策略[J].中国医药导报，2016，13（29）：37-40.
[15] Subburaman S，Ganesan K，Ramachandran M. Protective role of naringenin against doxorubicin-induced cardiotoxicity in a rat model：histopathology and mRNA expression profile studies [J]. J Environ Pathol Toxicol Oncol，2014， 33（4）：363-376.
[16] Liu Y，An BS，Gao A. Protective effects of naringenin in cardiorenal syndrome [J]. J Surg Res，2016，203（2）：416-423.
[17] Gürsul C，Ekinci Akdemir FN，Akkoyun T，et al. Protectice effect of Naringin on experimental hindlimb ischemia/reperfusion injury in rats [J]. J Enzyme Inhib Med Chem，2016，31（sup1）：56-61.
[18] Ibanez B，Fuster V，Jimenez-Borreguero J，et al. Lethal myocardial reperfusion injury：a necessary evil [J]. Int J Cardiol，2011，151（1）：3-11.
[19] 谭玉婷，鲁卫星，赵俊男，等.龙牙楤木总皂苷对缺血再灌注损伤大鼠心肌细胞线粒体细胞色素C和膜电位的影响及其作用机制[J].西部中医药，2016，29（12）：9-12.
[20] 佟彤，刘红旭，尚菊菊，等.参元丹对缺血再灌注心肌细胞内质网应激相关因子GRP78、CHOP表达的影响[J].世界中医药，2016，11（3）：407-409.
[21] Qin H，Walcott GP，Killingsworth CR，et al. Impact of myocardial ischemia and reperfusion on ventricular defibrillation patterns，energy requirements，and detection of recovery [J]. Circulation，2002，105（（21）：2537-2542.
[22] Wang HY，O'Doherty GA. Modulators of Na/K-ATPase：a patent review [J]. Expert Opin Ther Pat，2012，22（6）：587-605.
[23] Gadeberg HC，Bryant SM，James AF，et al. Altered Na/Ca exchange distribution in ventricular myocytes from failing hearts [J]. Am J Physiol Heart Circ Physiol，2016，310（2）：H262-H268.
[24] 姚期，余资江，孙宝飞，等.松树皮提取物对小鼠脑缺血再灌注损伤的保护作用及机制研究[J].中国实验动物学报，2017，25（6）：632-636.
[25] 程永生，陈宇，李冬.钙超载及其在心肌缺血/再灌注损伤中的作用机制研究现状[J].中国医学创新，2013，10（3）：150-151. |
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