基于转录组测序技术初步筛选内蒙古特禀体质人群的差异表达基因

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Abstract Objective To study the differential gene expression in the special constitution and the moderate constitution of Inner Mongolia by using the transcriptome sequencing technology, in order to explore the genesis, characteristics and related mechanisms of allergic diseases from the molecular level. Methods From September 2015 to September 2016, the Affiliated Hospital of Inner Mongolia Medical University, Tongliao City Hospital and the Physical Examination Center of Bayanur Hospital were investigated by epidemiology. The special constitution and the moderate constitution of the three generations of ancestors living in Inner Mongolia were selected as the research objects, the peripheral blood of 10 subjects were taken for transcriptome sequencing. Group sequencing and qPCR were used to verify the correlation between some differentially expressed genes and their related pathways and special constitution. Results Compared with moderate constitution, among all down-regulated genes in special constitution, HLA-DRB1, HLA-DRB5 and HLA-DQA2 were enriched in the asthma pathway; Toll like receptor 2 (TLR2) differentially expressed genes were enriched in the TLR signaling pathway, which was closely related to the special constitution. The qPCR test showed that the relative expression of CPNE3 genes in the special constitution was higher than that in the moderate constitution, the difference was statistically significant (P ＜ 0.05). Conclusion HLA class Ⅱ genes are closely related to special constitution and differentially express in the special constitution; TLR signaling pathway involved by gene TLR2 reflects that the signal transduction mechanism of allergic diseases may be closely related to special constitution; CPNE3 gene expression differences in special constitution and moderate constitution, indicating that there may be some differences in the expression of the two constitutions, but further studies are needed.

Key words： Transcriptome sequencing technology Special constitution Expressed gene

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	XUE Ziping KANG Lujiao ZHANG Yajun

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XUE Ziping KANG Lujiao ZHANG Yajun. Preliminary screening of differentially expressed genes in Inner Mongolia special constitutions based on transcriptome sequencing[J]. 中国医药导报, 2018, 15(35): 8-11,16.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I35/8

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