临床腹泻患者艰难梭菌的感染情况和分子流行特征

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Abstract Objective To understand the infection conditions and molecular epidemic characteristics of Clostridium difficile in clinical patients with diarrhea, to provide a basis for controlling nosocomial infections and clinical diagnosis and treatment. Methods Eight hundred and fifty-two feces samples from clinical diarrhea patients in the Second Hospital of Jiaxing City from January to December 2016 were collected as experimental group and 310 feces samples from healthy examination adults in the same period were collected as control group. The positive rate of Clostridium difficile was obtained by anaerobic culture and taken comprehensive analysis by combining with toxin genotype and multilocus sequence typing. Results A total of 108 Clostridium difficile were isolated from 852 feces samples of experiment group, with a positive rate of 12.68%, while a total of 8 Clostridium difficile were isolated from 310 feces samples of control group, with a positive rate of 2.58%, the difference between the two groups was statistically significant (P ＜ 0.05). The experiment group was dominated by tcdA+tcdB+ strains, accounted for 86.11% (93/108), tcdA-tcdB+ strains accounted for 5.56% (6/108), and tcdA-tcdB-strains accounted for 8.33% (9/108), the rate of toxin production was 91.67%, while the corresponding toxin genotypes of the control group accounted for 62.50% (5/8), 12.50% (1/8), 25.00% (2/8), and the rate of toxin production was 75.00%, the rate of toxin production between the two groups had no statistically significant difference (P ＞ 0.05). In the experiment group, 32 ST types were found by multisite sequence typing, among which, ST54 was a relatively popular type, while CC48 was a relatively popular clonal complex. Conclusion The infection rate of Clostridium difficile in clinical patients with diarrhea is high, which is urgent to attract clinical attention and strengthen the prevention and control of the hospital infection, to prevent epidemic outbreak.

Key words： Clostridium difficile Toxin genotype Multilocus sequence typing eBURST analysis

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	FENG Xuejun1 XU Shaoyi2 WU Xiaoyan1

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FENG Xuejun1 XU Shaoyi2 WU Xiaoyan1. The Clostridium difficile infection conditions and molecular epidemic characteristics of clinical diarrhea patients[J]. 中国医药导报, 2018, 15(34): 161-164.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I34/161

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