|
|
|
| Effects of curcumin on the expression of PTP1B and IRS-2 mRNA in liver tissue of rats with type 2 diabetes mellitus |
| QIN Peijie1 ZHANG Dongwei2 GAO Sihua2▲ LIU Jianfeng1▲ |
1.Institute of Chinese Medical History Literature, China Academy of Chinese Medical Sciences, Beijing 100700, China;
2.Diabetes Research Center, Beijing University of Chinese Medicine, Beijing 100029, China |
|
|
|
Abstract Objective To observe the effects of curcumin on expression of protein tyrosine phosphatase 1B (PTP1B) and insulin receptor substrate-2 (IRS-2) mRNA in liver tissue of rats with type 2 diabetes mellitus induced by high fat diet. Methods The SD rats were fed with high fat diet, then the tail vein was injected with Streptozotocin (STZ), so as to make type 2 diabetes mellitus model. The model rats were divided into model group, crucumin group, Pioglitazone group, with 10 rats in each group. After 4 weeks and 8 weeks of administration, the levels of fasting blood-glucose (FBG), fasting insulin (FINS), serum total cholesterol (TC), triacylglycerol (TG), low density lipoprotein cholesterin (LDL-C), high density lipoprotein cholesterol (HDL-C) were detected, and the liver index (LI), insulin sensitivity index (ISI) were calculated. After 8 weeks of administration, the pathological changes were observed by HE staining of liver, the mRNA expression of PTP1B and IRS-2 in liver was detected by real-time PCR. Results Compared with model group, crucumin group could significantly reduce the weight and the serum levels of FBG, FINS, LDL-C of rats with type 2 diabetes mellitus (P ＜ 0.05), increase the insulin sensitivity, improve the fatty degeneration and inflammatory reactions of liver tissue, inhibit the expression of PTP1B mRNA, and up-regulate the expression of IRS-2 mRNA (P ＜ 0.05). Conclusion Curcumin can improve the insulin resistance of model rats with type 2 diabetes mellitus, the mechanism may be related to inhibition of PTP1B mRNA expression and up-regulation of IRS-2 mRNA expression.
|
|
|
|
|
|
[1] 贾绍华，张舜尧.姜黄素药理作用研究进展[J].中国现代药物应用，2009，3（22）：188-189.
[2] 陈奇.中药药理研究方法[M].3版.北京：人民卫生出版社，2011：33.
[3] Withers DJ，White M. Perspective：the insulin signaling system--a common link in the pathogenesis of type 2 diabetes [J]. Endocrinology，2000，141（6）：1917-1921.
[4] Ide T，Shimano H，Yahagi N，et al. SREBPs suppress IRS-2-mediated insulin signalling in the liver [J]. Nat Cell Biol，2004，6（4）：351-357.
[5] Klaman LD，Boss O，Peroni OD，et al. Increased energy expenditure，decreased adiposity，and tissue-specific insulin sensitivity in protein-tyrosine phosphatase 1B-deficient mice [J]. Mol Cell Biol，2000，20（15）：5479-5489.
[6] 龚燕冰，罗增刚，高思华.运用因子分析方法探索2型糖尿病证候要素及其靶位特征[J].中医杂志，2011，52（13）：1100-1102.
[7] 高思华.以中西医结合理论为指导，立足肝脾肾辨治糖尿病[J].中国中西医结合杂志，1994，14（10）：622-623.
[8] Bengmark S. Curcumin，an atoxic antioxidant and natural NFκB，cyclooxygenase-2，lipooxygenase，and inducible nitric oxide synthase inhibitor：a shield against acute and chronic diseases [J]. JPEN，2006， 30（1）：45-51. |
|
|
|
