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| Effects of curcumin on apoptosis after human cytomegalovirus infection in human embryonic pulmonary fibroblasts |
| JIA Yangjie LYU Yali LIU Lihong |
| Department of Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China |
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Abstract Objective To investigate the apoptosis of human embryonic lung fibroblast cells infected with human cytomegalovirus (HCMV) and the effects of curcumin on apoptosis after HCMV infection. Methods The cells were divided into cell control group (cell maintenance solution), virus model group (cell maintenance solution + HCMV), high dose (cell maintenance solution + HCMV +0.8 μg/mL of curcumin ), middle dose (cell maintenance solution + HCMV + 0.4 μg/mL of curcumin) and low dose (cell maintenance solution + HCMV + 0.2 μg/mL of curcumin) of curcumin group, Ganciclovir group (cell maintenance solution + HCMV + 50 μg/mL of Ganciclovir). The effects of curcumin on apoptosis of HCMV infection were detected by mitochondrial membrane potential detection kit (JC-1) and fluorescent dye propidium iodide (PI) method. Results The JC-1 experimental results show that in the early stage of HCMV infection, there was no significant difference on cell apoptosis between virus model group and cell control group (P > 0.05); compared with virus model group, the apoptosis of Ganciclovir group and high dose, middle dose and low dose of curcumin group increased significantly (P < 0.05). In the late stage of HCMV infection, compared with cell control group, the apoptosis of virus model group increased significantly (P < 0.05); compared with virus model group, the cell apoptosis of high dose, middle dose and low dose of curcumin group decreased in different degrees, among them, the difference between the high dose of curcumin group and the virus model group was statistically significant (P < 0.05). The fluorescent dye PI results showed that in the early stage and late stage of HCMV infection, compared with cell control group, the number of host cells in the S phase in virus model group increased significantly (P < 0.01); compared with virus model group, the number of host cells in S phase in high dose, middle dose and low dose of curcumin group decreased significantly (P < 0.05). Conclusion Curcumin has antiapoptotic activity in HCMV infection and can weaken anti-apoptosis induced by early HCMV infection and pro-apoptosis induced by late HCMV infection.
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