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| Effects of amentoflame on proliferation and proliferation-related protein expression of neuroblastoma cells in mice |
| ZHU Pingping1 YUAN Wei2 WANG Pengzhen3 |
1.Department of Neurology, Guangzhou Red Cross Hospital of Jinan University, Guangdong Province, Guangzhou 510220, China;
2.Department of Surgery, Guangzhou Red Cross Hospital of Jinan University, Guangdong Province, Guangzhou 510220, China;
3.Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital of Jinan University, Guangdong Province, Guangzhou 510220, China |
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Abstract Objective To investigate the effects of amentoflame (AF) on proliferation and proliferation-related protein expression of neuroblastoma cells (Neuro-2a) in mice. Methods Neuro-2a cells were resuscitated, cultured, and selected for this study. The CCK-8 assay was divided into five groups: 0, 25, 50, 75 and 125 μmol/L groups, the cells were treated for 24 h. The effect of AF (75 μmol/L AF) on the percentage of G1 phase and S phase in the cell cycle of Neuro-2a was determined by cytometry. The effects of AF (75 μmol/L AF group) on the expression of proliferation-related mRNA and proteins in Neuro- 2a cells were detected by fluorescence quantitative PCR and Western blot. Results The results of CCK-8 showed that 25 and 50 μmol/L AF group had no significant effect on the activity of Neuro-2a cells (P＞0.05), while 75 μmol/L group AF significantly decreased the activity of Neuro-2a cells, and the difference was statistically significant (P＜0.05). Compared with the control group, the percentage of Neu-2A cells in G1 phase in 75 μmol/L AF group was significantly increased, and the difference was statistically significant (P＜0.05); compared with the control group, the percentage of S-phase cells of Neuro-2a cells in 75 μmol/L AF group was significantly decreased, and the difference was statistically significant (P＜0.05); however, 75 μmol/L AF group had no significant effect on the cell distribution in G2 phase of Neuro-2a cells. Quantitative PCR and Western blot results showed that 75 μmol/L AF inhibited the expression of β-catenin, CyclinD1, CyclinE, and Survivin mRNA and protein in Neuro-2a cells, and promote expression of p21 and p16 mRNA and protein (P＜0.05). Conclusion 75 μmol/L AF inhibited the proliferation of Neuro-2a cells by inhibiting the expression of β-catenin, CyclinD1, CyclinE, and Survivin, promoting the expression of proliferation-related proteins such as p21 and p16.
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[1] Han Y，Ye X，Wang C，et al. Integration of molecular features with clinical information for predicting outcomes for neuroblastoma patients [J]. Biol Direct，2019，14（1）：16.
[2] 王荣，张旭铭，敬晓莉，等.miR-186-5p通过下调MYCN轴增强小儿神经母细胞瘤NB1/DDP细胞顺铂敏感性的机制研究[J].沈阳药科大学学，2022，39（12）：1478-1485.
[3] Lee WP，Lan KL，Liao SX，et al. Inhibitory Effects of Amentof- lavone and Orobol on Daclatasvir-Induced Resistance-Associated Variants of Hepatitis C Virus [J]. Am J Chin Med，2018，46（4）：835-852.
[4] 傅娟，刘媛媛，李霞.穗花杉双黄酮抑制小儿血管瘤内皮细胞体外增殖实验研究[J].陕西医学杂志，2021，50（8）：937-940，965.
[5] Lin CH，Lin KH，Ku HJ，et al. Amentoflavone induces caspase-dependent/-independent apoptosis and dysregulates cyclin-dependent kinase-mediated cell cycle in colorectal cancer in vitro and in vivo[J]. Environ Toxicol，2023，38（5）：1078-1089.
[6] Chen WT，Chen CH，Su HT，et al. Amentoflavone Induces Cell-cycle Arrest，Apoptosis，and Invasion Inhibition in Non-small Cell Lung Cancer Cells [J]. Anticancer Res，2021， 41（3）：1357-1364.
[7] Chen Y，Li N，Wang H，et al. Amentoflavone suppresses cell proliferation and induces cell death through triggering autophagy-dependent ferroptosis in human glioma [J]. Life Sci，2020，247：117425.
[8] 黄佳，潘玫，汪利群，等.丙泊酚抑制wnt/β-catenin通路对宫颈癌HELA细胞生长和运动能力的调节作用[J].中国免疫学杂志，2020，36（23）：2861-2865，2871.
[9] Li F，Zhang L，Li W，et al. Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/β-catenin pathway [J]. Oncotarget，2015，6（8）：6001-6013.
[10] Kang J，Wang J，Cheng J，et al. Down-regulation of NTCP expression by cyclin D1 in hepatitis B virus-related hepatocellular carcinoma has clinical significance [J]. Oncotarget，2016，8（34）：56041-56050.
[11] 李腊秀，王科，赵家宁，等.胃癌组织SOX1和β-catenin的表达及其与临床病理特征、远期预后的相关性[J].中国现代医学杂志，2021，31（13）：30-34.
[12] Zhang Q，Sakamoto K，Wagner KU. D-type Cyclins are important downstream effectors of cytokine signaling that regulate the proliferation of normal and neoplastic mammary epithelial cells [J]. Mol Cell Endocrinol，2014，382（1）：583- 592.
[13] Yang M，Zhong J，Zhao M，et al. Overexpression of nuclear apoptosis-inducing factor 1 altered the proteomic profile of human gastric cancer cell MKN45 and induced cell cycle arrest at G1/S phase [J]. PLoS One，2014，9（6）：e100216.
[14] Liang J，Pan Y，Zhang D，et al. Cellular prion protein promotes proliferation and G1/S transition of human gastric cancer cells SGC7901 and AGS [J]. FASEB J，2007，21（9）：2247-2256.
[15] Pal D，Sur S，Mandal S，et al. Prevention of liver carcinogenesis by amarogentin through modulation of G1/S cell cycle check point and induction of apoptosis [J]. Carcinogenesis，2012，33（12）：2424-2431.
[16] Lin SY，Xia W，Wang JC，et al. Beta-catenin，a novel prognostic marker for breast cancer：its roles in cyclin D1 expression and cancer progression [J]. Proc Natl Acad Sci USA，2000，97：4262-4256.
[17] Wang H，Wang H，Makki MS，et al. Overexpression of β- cat- enin and cyclinD1 predicts a poor prognosis in ovarian serous carcinomas [J]. Int J Clin Exp Pathol，2013，7（1）：264-271.
[18] Sherr CJ，Roberts JM. CDK inhibitors：positive and negative regulators of G1-phase progression[J]. Gene Dev，1999，13（12）：1501-1512.
[19] 逄丽丽，贺琳，宋婵婵，等.ERα特异性抑制剂MPP降低小鼠2-细胞胚G1/S期过渡相关因子Nanog、cyclin D1和cyclin E水平[J].中国组织化学与细胞化学杂志，2017，26（2）：104-108.
[20] Hashimoto T，Yanaihara N，Okamoto A，et al. Cyclin D1 predicts the prognosis of advanced serous ovarian cancer [J]. Exp Ther Med，2011，2：213-229.
[21] Shimizu Y，Murakami N，Mori T，et al. Clinical impact of p16 positivity in nasopharyngeal carcinoma [J]. Laryngoscope Investig Otolaryngol，2022，7（4）：994-1001.
[22] Kumari R，Jat P. Mechanisms of Cellular Senescence：Cell Cycle Arrest and Senescence Associated Secretory Phenotype [J]. Front Cell Dev Biol，2021，9：645593.
[23] 沙巴海提·吾斯曼，杨银银，刘志琴，等.Survivin对食管癌细胞Tak1、NF-κB表达调控以及细胞周期和凋亡的影响[J].山东医药，2023，63（1）：6-9.
[24] Martínez-Sifuentes MA，Bassol-Mayagoitia S，Nava-Hern- ández MP，et al. Survivin in Breast Cancer：A Review [J]. Genet Test Mol Biomarkers，2022，26（9）：411-421. |
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