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| Effect of Shenqi Zhilong Decoction on expression of FcγRⅠ， FcγRⅡB， and FcγRⅢA in renal tissue of membranous nephropathy model mice |
| XUE Piliang1 WANG Xiangwei1 LI Xingyu1 WANG Wenwen1 WANG Shun1 LIU Xing2 |
1.Department of Acupuncture and Moxibustion, Heilongjiang College of Traditional Chinese Medicine, Heilongjiang Province, Harbin 150036, China;
2.Department of Geriatric, Heilongjiang College of Traditional Chinese Medicine, Heilongjiang Province, Harbin 150036, China |
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Abstract Objective To explore the effect of Shenqi Zhilong Decoction on expression of FcγRⅠ, FcγRⅡB, and FcγRⅢA in renal tissues of membranous nephropathy (MN) model mice. Methods Sixty SPF grade ICR mice, six-week old and weig- hting (23±4) g, half male and half female, were divided into blank group (10 mice) and model group (50 mice). The model group mice were injected with cationic-bovine serum albumin to replicate MN mouse model. The 40 mice successfully modeled were divided into model group, Shenqilong Decoction high-, low-dose group (24, 12 g/kg) and Tripterygium Glycoside Tablets group (14 mg/kg) according to the random number table method, with 10 mice in each group. After four weeks of administration, the mice were killed. HE and Masson staining were used to observe the pathological changes of renal tissues; immunofluorescence method was used to observe the protein fluorescence intensity of FcγRⅠ, FcγRⅡB, and FcγRⅢA in renal tissues; Western blot was used to detect the protein expression of FcγRⅠ, FcγRⅡB, and FcγRⅢA in renal tissues; the mRNA expression of FcγRⅠ, FcγRⅡB, and FcγRⅢA in renal tissues was detected by RT-PCR. Results HE staining showed no significant abnormalities in the morphology and structure of the epithelial cells, basement membrane, renal tubules, and renal interstitium in blank group. In model group, the glomerular volume increased, the glomerular capillary loop dilated, blood cell overflow, the mesangial thickened, the cystic cavity enlarged, and the skin on the wall of the cystic cavity was severely damaged. Compared with model group, the glomerular volume decreased, the basement membrane thickened and the skin on the wall of the renal capsule was not damaged or partially damaged in Shenqi Zhilong Decoction high-dose group. Masson staining showed that the glomerular basement membrane was normal in the blank group. In model group, there was a large amount of subepithelial photophilin deposition, basal membrane matrix reaction, vacuol-like steatosis of renal tubules, and a large amount of renal interstitial lymphatic hyperplasia. Compared with model group, the lesion of each administration group was reduced, and the improvement was more obvious in Shenqi Zhilong Decoction high-dose group. Compared with blank group, the protein fluorescence of FcγRⅠ and FcγRⅢA in model group was enhanced, while the protein fluorescence of FcγRⅡB was weakened (P＜0.05); compared with model group, the protein fluorescence of FcγRⅠ and FcγRⅢA in Shenqi Zhilong Decoction high-dose group was decreased (P＜0.05), while the protein fluorescence of FcγRⅡB in Shenqi Zhilong Decoction high- and low-dose group was increased (P＜0.05); compared with Shenqi Zhilong Decoction low-dose group, the protein fluorescence of FcγRⅠ and FcγRⅢA in Shenqi Zhilong Decoction high-dose group was decreased, and the protein fluorescence of FcγRⅡB was increased (P＜0.05). Compared with blank group, the protein expressions of FcγRⅠ and FcγRⅢA were increased in model group, while the protein expression of FcγRⅡB was decreased (P＜0.05); compared with model group, the protein expressions of FcγRⅠ and FcγRⅢA in Shenqi Zhilong Decoction high-dose group were decreased, while the protein expressions of FcγRⅡB in Shenqi Zhilong Decoction high- and low-dose groups were increased (P＜0.05); compared with Shenqi Zhilong Decoction low-dose group, the protein expression of FcγRⅡB in Shenqi Zhilong Decoction high-dose group was increased, and the protein expression of FcγRⅢA was decreased (P＜0.05). Compared with blank group, the mRNA expressions of FcγRⅠ and FcγRⅢA in model group were increased, while the mRNA expressions of FcγRⅡB was decreased (P＜0.05); compared with model group, the mRNA expressions of FcγRⅠ and FcγRⅢA in Shenqi Zhilong Decoction high- and low-dose groups were decreased, and the mRNA expression of FcγRⅡB was increased (P＜0.05). compared with Shenqi Zhilong Decoction low-dose group, the mRNA expressions of FcγRⅠ and FcγRⅢA in Shenqi Zhilong Decoction high-dose group were decreased, while the mRNA expression of FcγRⅡB were increased (P＜0.05). Conclusion The therapeutic effect of Shenqi Zhilong Decoction on MN mice is related to improving the pathological changes of renal tissues, reducing the expressions of FcγRⅠ and FcγRⅢA, and increasing the protein expression of FcγRⅡB, and the effect of Shenqi Zhilong Decoction with high concentration is better.
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