|
|
|
| Expression of acidic ribosomal phosphoprotein P2 in hepatocellular carcinoma and its influence on prognosis |
| YANG Qingqing HUANG Yang CHEN Jin XIAO Xueyi WU Xudong▲ |
| Department of Gastroenterology, Yancheng Clinical College of Xuzhou Medical University Yancheng NO.1 People’s Hospital, Jiangsu Province, Yanchang 224000, China |
|
|
|
|
Abstract Objective To investigate the expression of acidic ribosomal phosphoprotein P2 (RPLP2) in hepatocellular carcinoma (HCC) and its influence on prognosis. Methods The expression of RPLP2 in HCC tissues and its relationship with overall survival (OS) and disease-free survival (DFS) were analyzed by TCGA database. Cancer tissues and adjacent tissues of 60 HCC patients treated in Yancheng NO.1 People’s Hospital, Jiangsu Province from June 2018 to June 2022 were selected and the expression level of RPLP2 were detected by Western blot. According to the immunohistochemical staining score of RPLP2 in the cancer tissues, the patients were divided into high expression group (51 cases) and low expression group (nine cases). Survival curve and Cox regression model were used to analyze the expression of RPLP2 and its influence on the prognosis of HCC. Results According to the results of database analysis, the expression of RPLP2 in HCC cancer tissues was significantly up-regulated, and the OS and DFS in patients with high RPLP2 expression were shorter than those with low RPLP2 expression, the differences were statistically significant (P＜0.05). The expression levels of RPLP2 protein in cancer tissues of 60 HCC patients were higher than those in adjacent tissues, and the differences were statistically significant (P＜0.05). OS and DFS in high expression group were shorter than those in low expression group, and the differences were statistically significant (P＜0.05). Cox regression model analysis showed that TNM stage, Barcelona clinical liver cancer stage, Edmondson grade, alpha-fetoprotein, and RPLP2 expression levels were influencing factors for OS and DFS in HCC patients (P＜0.05). Conclusion RPLP2 is highly expressed in HCC tissues and is closely related to the clinical prognosis of patients.
|
|
|
|
|
|
[1] Bray F，Ferlay J，Soerjomataram I，et al. Global cancer statistics 2018：GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin，2018，68（6）：394-424.
[2] Wang W，Wei C. Advances in the early diagnosis of hepatocellular carcinoma [J]. Genes Dis，2020，7（3）：308-319.
[3] Luo P，Wu S，Yu Y，et al. Current Status and Perspective Biomarkers in AFP Negative HCC：Towards Screening for and Diagnosing Hepatocellular Carcinoma at an Earlier Stage [J]. Pathol Oncol Res，2020，26（2）：599-603.
[4] Liu Z，Pu Y，Bao Y，et al. Investigation of Potential Molecular Biomarkers for Diagnosis and Prognosis of AFP-Negative HCC [J]. Int J Gen Med，2021，14：4369-4380.
[5] Bruix J，Han KH，Gores G，et al. Liver cancer：Approaching a personalized care [J]. J Hepatol，2015，62（1 Suppl）：S144- S156.
[6] Lin Z，Li H，He C，et al. Metabolomic biomarkers for the diagnosis and post-transplant outcomes of AFP negative hepatocellular carcinoma [J]. Front Oncol，2023，13：1072775.
[7] Jang GY，Kim YS，Lee SE，et al. Improvement of DC-based vaccines using adjuvant TLR4-binding 60S acidic ribosomal protein P2 and immune checkpoint inhibitors [J]. Cancer Immunol Immunother，2021，70（4）：1075-1088.
[8] Kang J，Brajanovski N，Chan KT，et al. Ribosomal proteins and human diseases：molecular mechanisms and targeted therapy [J]. Signal Transduct Target Ther，2021，6（1）：323.
[9] Azevedo ALK，Gomig THB，Giner IS，et al. Comprehensive analysis of the large and small ribosomal proteins in breast cancer：Insights on proteomic and transcriptomic expression patterns，regulation，mutational landscape，and prognostic significance [J]. Comput Biol Chem，2022，100：107746.
[10] Kim HJ，Maiti P，Barrientos A. Mitochondrial ribosomes in cancer [J]. Semin Cancer Biol，2017，47：67-81.
[11] Artero-Castro A，Castellvi J，García A，et al. Expression of the ribosomal proteins Rplp0，Rplp1，and Rplp2 in gynecologic tumors [J]. Hum Pathol，2011，42（2）：194-203.
[12] Yuan H，Tu S，Ma Y，et al. Downregulation of lnc RNA RPLP 0P2 inhibits cell proliferation，invasion and migration，and promotes apoptosis in colorectal cancer [J]. Mol Med Rep，2021， 23（5）：309.
[13] 中华人民共和国国家卫生健康委员会医政医管局.原发性肝癌诊疗规范（2019年版）[J].肝癌电子杂志，2020， 7（1）：5-23.
[14] 日西丹·艾买提，艾尼瓦尔·艾木都拉，艾克拜尔·艾尼瓦尔，等.PPARα在肾透明细胞癌组织中的表达及其与预后的关系[J].现代肿瘤医学，2022，30（7）：1238-1243.
[15] Johnson P，Berhane S，Kagebayashi C，et al. Impact of disease stage and aetiology on survival in hepatocellular carcinoma：implications for surveillance [J]. Br J Cancer，2017， 116（4）：441-447.
[16] Xing M，Wang X，Kiken RA，et al. Immunodiagnostic Biom- arkers for Hepatocellular Carcinoma（HCC）：The First Step in Detection and Treatment [J]. Int J Mol Sci，2021，22（11）： 6139.
[17] Yang JD，Harmsen WS，Slettedahl SW. Factors that affect risk for hepatocellular carcinoma and effects of surveillance [J]. Clin Gastroenterol Hepatol，2011，9（7）：617-623.e1.
[18] Singal AG，Lampertico P，Nahon P. Epidemiology and sur- veillance for hepatocellular carcinoma：New trends [J]. J Hepatol，2020，72（2）：250-261.
[19] Maehama T，Nishio M，Otani J，et al. Nucleolar stress：Molecular mechanisms and related human diseases [J]. Cancer Sci，202，114（5）：2078-2086.
[20] El Khoury W，Nasr Z. Deregulation of ribosomal proteins in human cancers [J]. Biosci Rep，2021，41（12）：BSR20211577.
[21] de Las Heras-Rubio A，Perucho L，Paciucci R，et al. Ribosomal proteins as novel players in tumorigenesis [J]. Cancer Metastasis Rev，2014，33（1）：115-141.
[22] Therizols G，Bash-Imam Z，Panthu B，et al. Alteration of ribosome function upon 5-fluorouracil treatment favors cancer cell drug-tolerance [J]. Nat Commun，2022，13（1）：173.
[23] Cai Z，Wu Y，Zhang F，et al. A three-gene signature and clinical outcome in pediatric acute myeloid leukemia [J]. Clin Transl Oncol，2021，23（4）：866-873.
[24] Pecoraro A，Pagano M，Russo G，et al. Ribosome Biogenesis and Cancer：Overview on Ribosomal Proteins [J]. Int J Mol Sci，2021，22（11）：5496. |
|
|
|
