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| The preventive effect of Huoxue Jiangtang Decoction on pancreatic β cell apoptosis and its influence on scrib protein expression in type 2 diabetes rats |
| LIU Deliang1 LI Huilin1 QU Xin1 ZHAO Hengxia1 CHU Shufang1 LIU Xuemei1 ZENG Lin2 ZHANG Xuewen2 |
1.Department of Endocrinology, Shenzhen Traditional Chinese Medicine Hospital, Guangdong Province, Shenzhen 518033, China;
2.the Fourth Clinical Medical College, Guangzhou University of Chinese Medicine, Guangdong Province, Shenzhen 518033, China;
3.Institute of Traditional Chinese Medicine Master, Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712046, China |
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Abstract Objective To investigate the preventive effect of Huoxue Jiangtang Decoction on pancreatic β cell apoptosis and its influence on scrib protein expression in type 2 diabetes mellitus rats. Methods The type 2 diabetes mellitus model was induced by high fat and high calorie diet and intravenous injection of low dose of Streptozotocin. By SPSS software, all model rats were divided into model group, herbal group, Metformin group, besides, normal group was added, 10 rats in each group. After treatment, the blood glucose, insulin and lipids were tested; the pancreatic β cell apoptosis was examined by terminal-deoxynucleoitidyl transferase mediated nick end labeling method; and scrib protein expression was detected by Western blot in extracted and purified pancreatic islet cells. Results Compared with model group, the levels of fasting blood-glucose, 2 h postprandial blood glucose, fasting insulin, 2 h postprandial insulin, insulin resistance index and total cholesterol, triacylglycerol, low density lipoprotein cholesterin in herbal group and Metformin group were all decreased, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01), while high density lipoprotein cholesterol had no obvious changes, the difference was not statistically significant (P ＞ 0.05). Compared with model group, the β cell apoptosis indices in herbal group and Metformin group were also decreased (P ＜ 0.01), meanwhile, the scrib protein expression in islet cells was increased significantly (P ＜ 0.01). Conclusion Huoxue Jiangtang Decoction treatment has the effects of regulating blood lipid, decreasing blood glucose, improving insulin resistance, which can effectively prevent β cell apoptosis. The mechanism may be related to the upregulation of scrib protein expression in islet cells.
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