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| Expression of lncRNA XIST and miR-132-3p in endometrial cancer tissues and the relationship with pathological parameters and prognosis |
| REN Xiaoxia1 XU Haibo2 WU Jinting3 WANG Chenyi4 HAN Yaqin1 |
1.Department of Obstetrics and Gynecology, the Sixth People’s Hospital of Nantong Affiliated Nantong Hospital of Shanghai University, Jiangsu Province, Nantong 226000, China;
2.Department of Gynecology, Nantong Tumor Hospital, Jiangsu Province, Nantong 226000, China;
3.Department of Gynecology, Nantong Maternal and Child Health Care Hospital, Jiangsu Province, Nantong 226000, China;
4.Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Jiangsu Province, Nantong 226000, China |
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Abstract Objective To investigate the expression of long non-coding RNA X inactive specific transcript (lncRNA XIST), microRNA-132-3p (miR-132-3p) in endometrial cancer (EC) tissues and the relationship with pathological parameters and prognosis. Methods A total of 100 EC patients admitted to the Sixth People’s Hospital of Nantong and Nantong Tumor Hospital, Jiangsu Province from February 2016 to December 2019 were selected. qPCR was used to detect the expression of lncRNA XIST and miR-132-3p in cancer and paracancer tissues. The StarBase database predicted the relationship between lncRNA XIST and miR-132-3p, and Pearson correlation analysis was used to correlate the expression of lncRNA XIST and miR-132-3p. At three years follow-up, according to the average expression of lncRNA XIST and miR-132-3p in EC tissues, the patients were divided into high expression group and low expression group, and the survival curves of patients with different lncRNA XIST and miR-132-3p expression in EC tissues were plotted using the K-M method. Results lncRNA XIST expression in cancer tissue was higher than that in paracancerous normal tissue, and miR-132-3p expression was lower than that in paracancerous normal tissue (P＜0.05). There was a complementary sequence between lncRNA XIST and miR-132-3p, lncRNA XIST was negatively correlated with miR-132-3p expression in EC tissues (r =-0.694, P＜0.01). The expression of lncRNA XIST and miR-132-3p in EC tissues of different differentiation degrees, International Federation of Obstetrics and Gynecology (FIGO) stage, vascular invasion, and lymph node metastasis were statistically significant (P＜0.05). The 3-year cumulative survival rate of the lncRNA XIST high expression group was lower than that of the lncRNA XIST low expression group, and the 3-year cumulative survival rate of the miR-132-3p high expression group was higher than that of the miR-132-3p low expression group (P＜0.05). Conclusion The expression of lncRNA XIST is high and that of miR-132-3p is low in EC tissue, and its correlate with differentiation degree, FIGO stage, vascular invasion, and lymph node metastasis and prognosis.
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