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| Meta-analysis of methylene tetrahydrofolate reductase gene C677T polymorphism and susceptibility of non-alcoholic fatty liver disease |
| TANG Jin1 LI Xinghai2 ZHANG Ning1 |
1.Department of Gastroenterology, Ganzhou People’s Hospital, Jiangxi Province, Ganzhou 341000, China;
2.Department of Minimally Invasive Intervention, Ganzhou People’s Hospital, Jiangxi Province, Ganzhou 341000, China |
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Abstract Objective To investigate the relationship between C677T polymorphism of methylene tetrahydrofolate reductase (MTHFR) gene and susceptibility of non-alcoholic fatty liver disease (NAFLD). Methods PubMed, Cochrane Library, Wanfang Data, CNKI, China Biomedical Literature Database, and VIP were searched by computer to collect literatures on the association between MTHFR gene polymorphism and NAFLD susceptibility. The search time was from the inception to March 2023. In the T vs C, TT vs CC, TT vs CT+CC, TT+CT vs CC, CT vs CC, and TT vs CT gene models, the OR value and 95%CI were used as effect indicators for meta-analysis, and the publication bias and sensitivity analysis were tested by Stata 12.0 software. Results A total of 13 literatures were included, including 5 542 patients with NAFLD and 3 699 controls. Meta-analysis results showed that the risk of NAFLD was increased in five genetic models (T vs C: OR=1.29, 95%CI[1.09-1.52], P=0.003; TT vs CC: OR=1.65, 95%CI[1.23-2.23], P=0.001; TT vs CT+CC: OR=1.48, 95%CI[1.15-1.91], P=0.003; CT+TT vs CC: OR=1.31, 95%CI[1.06-1.62], P=0.013; TT vs CT: OR=1.40, 95%CI[1.09-1.80], P=0.009), heterozygote model CT vs CC was not associated with the risk of NAFLD (OR=1.21, 95%CI[0.98-1.48], P=0.070). The Egger test and funnel plot results showed no publication bias (P＞0.05). Meta-regression analysis and sensitivity analysis did not find significant sources of heterogeneity (P＞0.05). Conclusion The polymorphism of MTHFR gene C667T is associated with NAFLD, and TT genotype has an increased risk of NAFLD.
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