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| Effect of electroacupuncture preconditioning on the expression of mitop-hagy related proteins in myocardial ischemia-reperfusion injury rats |
| TANG Qiang1 MA Xiyuan1 LIU Xinxin1 YIN Xia1 ZHAO Yaowei1 LI Yuting1 MENG Xianghong1 LI Hongyu2▲ |
1.Second Clinical Medical College, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
2.Department of Rehabilitation, the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150001, China |
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Abstract Objective To investigate the effect of electroacupuncture preconditioning on the expression of autophagy-related proteins in myocardial ischemia-reperfusion injury rats’ mitochondria. Methods Thirty-six male SD rats, aged 8-10 weeks and with body weight 180-220 g were divided into control group, model group, and electroacupuncture pretreatment group by random number table method. The rat myocardial ischemia-reperfusion injury model was developed by an outside-in perfusion system. Rats in control group were only perfused for three hours. Rats in model group and electroacupuncture pretreatment group were perfused for 20 minutes，then ischemia for 40 minutes, and reperfusion for two hours. The electroacupuncture pretreatment group underwent electroacupuncture treatment for three weeks before modeling. The contents of creatine kinase (CK), creatine kinase (CK-MB), and lactate dehydrogenase (LDH) were detected in myocardial tissue; hematoxylin-eosin (HE) staining was stained to visualize the lesions in the groups of cardiomyocytes. Mitochondrial autophagy-related proteins Beclin-1, P62, PINK1, and Parkin exposure were identified by Western blot. Results The contents of CK, CK-MB, and LDH in the model group were higher than those in the control group, with statistical significances (P＜0.05); the contents of CK，CK-MB, and LDH in the electroacupuncture pretreatment group were less than those in the model group, with statistical significances (P＜0.05). HE staining results indicated that cardiac muscle fibers in the model group were mostly fractured, degenerated, necrotic, and arranged loosely and irregularly.The myocardial tissue structure in the electroacupuncture pretreatment group was basically intact, the intercellular edema was mild, the muscle fiber space was slightly widened, and the necrotic focus was lightened. The levels of mitochondrial autophagy marker proteins Beclin-1, P62, PINK1, and Parkin in model group were higher than those in control group, with statistical significances (P＜0.05). The levels of mitochondrial autophagy marker proteins Beclin-1, PINK1, and Parkin in electroacupuncture pretreatment group were higher than those in model group, and the level of P62 was lower than those in model group, with statistical significances (P＜0.05). Conclusion Electroacupuncture pretreatment can improve myocardial ischemia/reperfusion injury by regulating the expression of PINK1 and Parkin protein, and activating mitochondrial autophagy.
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