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| Diagnosis of invasive pulmonary aspergillosis by metagenomic next-generation sequencing: a case report |
| SUN Jie1 LIU Jian2 FENG Jihong2 |
1.Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
2.Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300143, China |
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Abstract Invasive pulmonary aspergillosis is difficult to distinguish from other pulmonary infections in clinic, and is easy to be misdiagnosed or missed. This paper report, a case of invasive pulmonary aspergillosis diagnosed by macrogene second generation sequencing, to investigate the diagnostic value of metagenomic next-generation sequencing in patients with invasive pulmonary aspergillosis. The main clinical manifestations of the patient were high fever, cough, and sputum with blood, after admission, the initial diagnosis was “bacterial pneumonia”, Cefoperazone sodium sulbactam sodium, Meropenem were given successively, the effect is not good, the relevant examination did not obtain sufficient etiological evidence, the metagenomic next-generation sequencing technology and pathological examination were performed, and the results indicated pulmonary infection of aspergillus, the antibiotic was changed to Voriconazole, and the patient was discharged after treatment. The possibility of fungal infection should be considered in patients with pulmonary infection who are not well treated by conventional antibiotics, if the pathogenic bacteria cannot be identified by routine examination, the diagnostic value of metagenomic next-generation sequencing technology should be emphasized.
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