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| Study on pharmacoynamics and safety of Shenbai-Qudu Teabag |
| JIA Fei1 WANG Xiaofeng1 LI Guodong1 WANG Yanyun2 PAN Jian3 LI E1 |
1.Department of Anorectal Medicine, Guang’anmen Hospital China Academy of Chinese Medical Science, Beijing 100053, China;
2.New Drug Research and Develope Center, Guang’anmen Hospital China Academy of Chinese Medical Science, Beijing 100053, China;
3.Department of Clinical Laboratory, Guang’anmen Hospital China Academy of Chinese Medical Science, Beijing 100053, China |
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Abstract Objective To observe the pharmacodynamics and safety indicators of Shenbai-Qudu Teabag. Methods A total of 60 SD rats (30 females and 30 males) with body weight of 150-170 g were divided into blank group, Shenbai high (1.050 g crude drug/ml), medium (0.525 g crude drug/ml), and low (0.263 g crude drug/ml) dose groups and Piyanping (0.600 g/ml) group according to random number table method, with 12 rats in each group. 0.1 ml of 1% carrageenan was injected subcutaneously into the left posterior foot of rats, the hourly plantar volume was measured between 1-5 h after administration, and the swelling degree was calculated. Forty-eight Kunming mice, females, weight 18-24 g, were divided into blank group, Shenbai high (1.750 g crude drug/ml), medium dose(0.875 g crude drug/ml), and low dose (0.438 g crude drug/ml) groups according to random number table method, with 12 cases in each group. The basal pain threshold of mice was determined, and then the mice were soaked in a drug bath at 40℃ for 20 minutes, respectively. The pain threshold of mice was measured at five time points after administration, and the percentage increase of pain threshold was calculated. A total of 30 male and female guinea pigs with body weight of 280-320 g were divided into blank group, 2, 4-dinitrochlorobenzene group, and ginseng and cypress group according to random number table method, with 10 guinea pigs in each group. 0.2 ml of Shenbai was taken and apply it to the right depilatory area for 6 h, and repeat once on the 7th and 14th day respectively. 14 days after the last administration, 0.2 ml of the drug was applied to the left depilatory area of guinea pigs, and the local skin and the whole body situation immediately and 24, 48, and 72 h for evaluation were observed. Results The swelling degree of foot in Shenbai high dose group and medium dose group at 5 h were lower than that at 3 h (P＜0.05). The foot swelling of Shenbai high dose group and medium dose group was lower than that of blank group at 2-3 h (P＜0.05), and the foot swelling of Shenbai low dose group was lower than that of blank group at 3 h (P＜0.05). The basal pain threshold of Shenbai high dose group for 0.5-4.5 h, medium dose group for 1.5-4.5 h and low dose group for 3.5-4.5 h were higher than basic pain threshold of this group(P＜0.05). The pain threshold of Shenbai high dose group was higher than that of blank group at 0.5 ~ 4.5 h (P＜0.05), and that of medium dose group at 1.5-3.5 h was higher than that of blank group (P＜0.05). Shenbai-Qudu Teabag had no sensitization effect. Conclusion Shenbai-Qudu Teabag has significant anti-inflammatory and analgesic effects when applied externally, and it is safe for topical use.
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