基于TLRs/NF-κB信号通路观察不同滑膜细胞来源炎性外泌体对家兔膝关节的影响及其作用机制

doi:10.20047/j.issn1673-7210.2023.27.02

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Abstract Objective To observe the effect of inflammatory exosomes derived from different synoviocytes on rabbit knee joint and its mechanism based on TLRs/NF-κB signal pathway. Methods Two synoviocytes were treated with lipopolysaccharide to induce inflammation, the exosomes were extracted separately. Twenty experimental rabbits were divided into five groups by random number table method: normal group, saline group, group A (injected with exosome suspension of A cell), group B (injected with exosome suspension of B cell), and group A+B (injected with two kinds of exosome mixed suspension), with four rabbits in each group. The normal group was not treated, the saline group was injected with normal saline, and the corresponding exosome suspension was injected into the joint cavity of rabbits in the exosome group (the concentration was 1×107/ml). After two weeks of intervention, the behavioral changes of rabbits in each group were recorded, and the expression levels of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), IL-6 and matrix metalloproteinase 13 (MMP-13) in serum were detected by enzyme-linked immunosorbent assay, Toll-like receptor 2 (TLR2), P65, myeloid differentiation factor 88 (MyD88) and TNF receptor-associated factor 2 (traf2) were detected by Western blot. Results There was no significant difference in behavioral Lequesne MG index between normal group and saline group before and after intervention (P＞0.05). The behavioral Lequesne MG index of rabbits in groups A, B and A+B after intervention was higher than that before intervention, and higher than that in normal group (P＜0.05); the levels of TNF-α, IL-1β, IL-6, and MMP-13 in serum and the expressions of TLR2, P65, MyD88, and traf2 in synovium tissue of rabbits in groups A, B and A+B were higher than those in normal group (P＜0.05); the levels of TNF-α, IL-1β, IL-6, and MMP-13 in serum of rabbits , and the levels of TLR2, P65, MyD88, and traf2 in synovium in group A+B were higher than those in groups A and B (P＜0.05). The protein expressions of TLR2, P65, MyD88, and traf2 in synovium tissue of rabbits in group A were higher than those in group B (P＜0.05). Conclusion Both kinds of synoviocytes secretions in inflammatory environment can activate synovial TLRs/NF-κB signal pathway aggravates synovial inflammation.

Key words： Fibroblast-like synoviocytes Macrophage-like synoviocytes Exosomes TLRs/NF-κB signal pathway Knee osteoarthritis

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	ZHOU Jun1 GUO Changqing2 WANG Qingfu3

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ZHOU Jun1 GUO Changqing2 WANG Qingfu3. Effect of inflammatory exosomes derived from different synoviocytes on rabbit knee joint and its mechanism based on TLRs/NF-κB signal pathway[J]. 中国医药导报, 2023, 20(27): 12-15.

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https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2023.27.02 OR https://www.yiyaodaobao.com.cn/EN/Y2023/V20/I27/12

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