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| Experimental study on long-term toxicity test of Scorzonera Herpes Zoster Ointment |
| HUANGFU Jiaxin1 CHU Xia2 QIN Zhen1 SHEN Dexin1 |
1.Department of Pediatrics, No.988 Hospital of Logistic Support Forces of the People’s Liberation Army, Henan Province, Zhengzhou 450042, China; 2.Department of Pathology, No.988 Hospital of Logistic Support Forces of the People’s Liberation Army, Henan Province, Zhengzhou 450042, China
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Abstract Objective To observe the long-term toxicity test of Scorzonera Herpes Zoster Ointment on New Zealand rabbits. Methods A total of 32 healthy and specific pathogen free New Zealand rabbits aged 10-14 weeks and weighing 2.0-2.5 kg, half male and half female were selected, and they were divided into control group, Scorzonera Herpes Zoster Ointment high dose group (2 g crude drug/kg), Scorzonera Herpes Zoster Ointment middle dose group (1 g crude drug/kg), and Scorzonera Herpes Zoster Ointment low dose group (0.5 g crude drug/kg), with eight rabbits with each group. The hairs on both sides of the spine were symmetrically removed in four groups, with a total of 150 cm2. Each group was given percutaneous administration for six days a week for four weeks, and the general situation was observed and recorded. After four weeks of administration, four New Zealand rabbits were selected from each group to detect hematological indexes and blood biochemical indexes; then the New Zealand rabbit was dissected, its organs were weighed, and the organ index was calculated; histopathological examination of the organs and the local skin were performed. The remaining animals were fed and recovered for two weeks after drug withdrawal, and the above indexes were detected. Results Four weeks after administration and two weeks after withdrawal, no obvious erythema, edema and other irritant reactions were found in four groups; the general condition of four groups was good, no abnormalities were found in drinking water, feeding, fur luster, activity, and bowel movements. The body weight increased gradually and the growth was good. There were no death or other toxic side effects caused by Scorzonera Herpes Zoster Ointment high, medium, and low dose groups. Four weeks after administration and two weeks after withdrawal, there were no significant differences in white blood cell count, neutrophil ratio, lymphocyte ratio, monocyte ratio, red blood cell count, hemoglobin, hematocrit, platelet count, prothrombin time, thrombin time, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, total protein, albumin, total bilirubin, alkaline phosphatase, potassium, sodium, chlorine, urea nitrogen, creatinine, creatine kinase, blood glucose, and the coefficients of liver, heart, spleen, lung, pancreas, and kidney among four groups (P＞0.05). Four weeks after administration and two weeks after withdrawal, the morphology, hardness, and color of organs among four groups were not abnormal due to drugs; no abnormal histological changes were observed in heart, liver, spleen, lung, kidney, adrenal gland, pancreas, brain, stomach, testis, small intestine, rectum, bladder, and thymus in each group by histopathology examination, and no edema, epidermal hyperplasia, and keratosis were found in skin histopathological examination. Conclusion There is no obvious toxic effect on New Zealand rabbits after long-term administration of Scorzonera Herpes Zoster Ointment.
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