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| Effect of Shenqi Jieyu Formula inhibiting inflammatory response in rats with postpartum depression |
| LI Jingya QIU Chao ZHANG Liping |
Department of Neurology, the First Affiliated Hospital of Zhejiang Chinese Medical University Zhejiang Provincial Hospital of Traditional Chinese Medicine, Zhejiang Province, Hangzhou 310000, China
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Abstract Objective To investigate the regulating effect of Shenqi Jieyu Formula on the inflammatory response of prefrontal microglia in rats with postpartum depression (PPD). Methods Twenty four two month old SD female rats were divided into sham operation group, PPD model group, Shenqi Jieyu Formula group and Western medicine control group by random number table method, with six animals in each group; the castrated rats were injected subcutaneously with 2.5 μg estradiol and 5 mg progesterone for 16 consecutive days, after seven days, 50 μg estradiol was injected daily, and the hormone injection was stopped on day 24. The PPD rat model was established by simulating the changes of estrogen during pregnancy and postpartum. Shenqi Jieyu Formula group was given Shenqi Jieyu Formula Granules dissolved in distilled water by intragastric administration. The Western medicine control group was given Paroxetine Hydrochloride by intragastric administration. Sham operation group and PPD model group were given 2 ml distilled water by intragastric administration. After three weeks of drug intervention, the open field experiment (cross grid number and upright time) were tested, and the dorsolateral prefrontal lobes of rats were selected to detect tumor necrosis factor-α(TNF-α), cortisol (Cor), and microglial marker Iba1 antibody levels. Results The number of horizontal bars and standing times in PPD model group were lower than those in sham operation group, and the levels of TNF-α, Cor, and Iba1 were higher than those in sham operation group, and the differences were statistically significant (P＜0.05); the number of horizontal bars and standing times in Shenqi Jieyu Formula group were higher than those in PPD model group, while the levels of TNF-α, Cor, and Iba1 were lower than those in PPD model group, and the differences were statistically significant (P＜0.05). Conclusion Shenqi Jieyu Formula can improve the behavior of PPD rats, inhibit the expression of microglia in prefrontal lobe of PPD rats, and reduce the inflammatory reaction and Cor level.
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