|
|
|
| Effect of three knee stitches on the morphological and behavioral changes of knee osteoarthritis model rat cartilage |
| LI Renzhong1 SUN Kui2 |
1.The Second Clinical Medical College, Anhui University of Chinese Medicine, Anhui Province, Hefei 230061, China;
2.Department of Orthopedics, the Second Affiliated Hospital of Anhui University of Chinese Medicine Anhui Acupuncture Hospital, Anhui Province, Hefei 230061, China
|
|
|
|
Abstract Objective To investigate the effect of three knee stitches on the morphological and behavioral changes of knee osteoarthritis (KOA) model rats. Methods A total of 28 male SPF SD rats (three to four weeks old, weight 180 to 220 g) were divided into blank group and model group with eight rats, acupuncture group with six rats, and western medicine group with six rats respectively by random number table method. The rat model of KOA was established by anterior cruciate ligament transection except the blank group. After five weeks of modeling, the acupuncture group received three knee stitches from the left knee joint for acupuncture treatment, once a day, and rested for five consecutive days for two days for three weeks. In the western medicine group, Diclofenac Diethylamine Latex was applied outside the affected knee. Blank group and model group were not involved. The morphological changes of cartilage after X-ray film, Lequesne MG score, Mankin’s score, cartilage, and HE staining were observed and compared. Results Compared with blank group, the left knee articular surface space narrowed, articular surface wear, and osteophyte formation were obvious in model group, articular cartilage surface defect was obvious, the number and distribution of cells were decreased, and Lequesne MG and Mankin’s scores were increased (P＜0.05). Compared with model group, Lequesne MG and Mankin’s scores in acupuncture group were decreased (P＜0.05), the surface of cartilage was slightly rough and shiny, and the number of cells was increased and arranged in a clear and orderly manner. The Lequesne MG scores in model group, acupuncture group and western medicine group after treatment were lower than those before treatment (P＜0.05). Conclusion Three knee stitches can improve the morphologic and behavioral indexes of the cartilage and protect the knee joint of KOA rats.
|
|
|
|
|
|
[1] 雷光华，王坤正.骨关节炎诊疗指南（2018年版）解读[J].中华骨科杂志，2018，38（12）：716-717.
[2] 李溪.膝关节骨性关节炎危险因素的病例对照研究[D].昆明：昆明医科大学，2019.
[3] 温菁婷.膝三针结合泰式按摩治疗中老年膝骨关节炎临床疗效观察[D].广州：广州中医药大学，2021.
[4] 潘丽，荆琳，王桂彬，等.膝骨关节炎的中西医研究进展[J].世界中医药，2022，17（16）：2373-2377.
[5] 陈益丹，邱华平，金肖青，等.不同针灸方法对膝骨关节炎模型细胞因子及基质金属蛋白酶影响的比较研究[J].中国比较医学杂志，2016，26（1）：42-45，64.
[6] 李忠仁.实验针灸学[M].北京：中国中医药出版社，2003：327-329.
[7] Lequesne MG，Samson M. Indices of severity in osteoarthritis for weight bearing joints [J]. J Rheumatol Suppl，1991，27（2）：16-18.
[8] 许波.阳和汤对兔膝骨关节炎模型IL-1、TNF-α含量影响的实验研究[D].福州：福建中医药大学，2016.
[9] 章晓云，曾浩，孟林.膝骨关节炎疼痛机制及治疗研究进展[J].中国疼痛医学杂志，2023，29（1）：50-58.
[10] 宋春波，王杰，蓝珍，等.壮药泥灸治疗膝骨性关节炎的临床观察[J].右江民族医学院学报，2022，44（3）：445-448.
[11] 李满意，刘红艳，陈传榜，等.骨痹的证治[J].风湿病与关节炎，2020，9（12）：53-56.
[12] 李毅，李盛华，周明旺，等.温针灸治疗早中期膝骨关节炎镇痛机制研究进展[J].中医临床研究，2022，14（24）：108-111.
[13] 胡斌.塞来昔布联合富血小板血浆对膝骨关节炎大鼠软骨组织形态学的影响[J].中国临床药理学杂志，2020， 36（14）：2017-2020.
[14] 林晴，王文义，潘丹虹，等.独活寄生汤对内质网应激下软骨细胞钙稳态和凋亡的影响[J].康复学报，2020，30（6）：468-473.
[15] 魏巍.补正续骨丸联合人工骨植骨修复ONFH的临床及实验研究[D].沈阳：辽宁中医药大学.
[16] 李辉，谢兴文，赵永利，等.中药有效成分防治骨关节炎的作用机制研究进展[J].中草药，2022，53（23）：7543- 7552.
[17] 芮靖琳，商志浩，黎柳娇，等.针刺对PERK-CHOP通路及胰腺组织中内质网应激的影响[J].中国老年学杂志，2022，42（12）：3034-3036.
[18] 吴浩，孟志超，曹永平，等.内质网应激对膝骨关节炎大鼠关节软骨细胞的影响[J].中国组织工程研究，2017， 21（16）：2502-2508.
[19] 吴双，邢金云，王燕，等.艾灸对膝骨关节炎模型兔软骨病理学改变及行为学改变的影响[J].中医药导报，2021， 27（1）：24-27.
[20] 李忠正，郭义，郭永明.浅谈针灸作用的基本特点[J].针灸临床杂志，2012，28（11）：5-6.
[21] 曹姚佳妮，黄琴峰，杨光，等.基于随机对照试验的针灸治疗膝骨关节炎临床规律分析[J].上海针灸杂志，2022， 41（10）：1032-1038.
[22] 薛玺情，李旭豪，马欣，等.膝姜灸治疗风寒湿痹型膝骨性关节炎的临床观察[J].时珍国医国药，2020，31（1）：137-139.
[23] 梁爱军，杨文慧，刘斌.膝三针治疗膝骨关节炎临床研究[J].中西医结合研究，2021，13（3）：166-169.
[24] 谭惠予，冯云亮.温针灸深刺犊鼻、内膝眼治疗膝关节骨性关节炎的临床疗效分析[J].中医临床研究，2016，8（14）：23-24.
[25] 孙奎，鲍学梅，宋阳春，等.针灸配合手法治疗膝骨性关节炎临床疗效评价[J].中医药临床杂志，2014，26（8）：817-819.
|
|
|
|
