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| Influence of Jiawei Danxuan Koukang on oral submucosal fibrosis and the level of autophagy in rats |
| HE Jun1* ZHANG Ting1* HU Liang1 PAN Shijie1 ZOU Hong1 LIAN Kequan1 TAN Jin2 HU Zhaoyong1▲ |
1.School of Medicine, Hunan University of Chinese Medicine, Hunan Province, Changsha 410208, China;
2.Department of Stomatology, the First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan Province, Changsha 410007, China
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Abstract Objective To investigate the influence of Jiawei Danxuan Koukang on oral submucous fibrosis (OSF) and the level of autophagy in rats. Methods Twenty-four SPF male SD rats (180-200 g, 4-8 weeks old) were randomly divided into blank group, model group, and Jiawei Danxuan Koukang group, with eight rats in each group. In the model group and Jiawei Danxuan Koukang group, arecoline 0.2 ml (10 mg/ml) was injected into the bilateral buccal mucosa of the rats, once every two days, and a small toothbrush was used to brush the bilateral buccal mucosa of the rats every day for eight weeks. White lesions were observed in the buccal mucosa of the rats to determine the success of the model. After the model was successfully established, the rats in the Jiawei Danxuan Koukang group were treated with 8 ml/(kg·d) Jiawei Danxuan Koukang by gavage, and the blank group and the model group were treated with the same amount of 0.9% sodium chloride solution by gavage for four weeks. After the experiment, the bilateral buccal mucosa of the three groups were collected for hema- toxylin-eosin staining and Masson staining to observe the pathological changes and collagen fiber deposition, and the number of autophagosomes in the three groups was observed by transmission electron microscope. Immunohistochemical staining was used to detect the expression of E-cadherin and microtubule- associated protein light chain 3 (LC3) positive cells in the three groups. Western blot was used to detect the expression of E-cadherin, LC3-Ⅱ, and selective autophagy adaptor protein p62. The mRNA expression of LC3-Ⅱ and p62 was detected by RT-PCR. Results In the model group, the oral mucosal epithelium was significantly atrophed, the collagen fibers were significantly thickened and increased, the epithelial layer was thinner, and a large amount of collagen fibers were deposited in the submucosal layer. In the Jiawei Danxuan Koukang group, the oral mucosal epithelium was thicker, the collagen fibers were reduced, and the atrophy of the epithelial layer was alleviated. In model group, the number of autophagosomes was lower than that in blank group, the number of E-cadherin and LC3 positive cells were lower than those in blank group, the expression of E-cadherin and LC3-Ⅱ protein were lower than those in blank group, the expression of LC3-Ⅱ mRNA was lower than that in blank group, and the expression of p62 protein and its mRNA were higher than those in blank group (P＜0.05). In Jiawei Danxuan Koukang group, the number of autophagosomes was higher than that in model group, the expression of E-cadherin and LC3-Ⅱ protein were higher than those in model group, the expression of LC3-Ⅱ mRNA was higher than that in model group, and the expression of p62 protein and its mRNA were lower than those in model group (P＜0.05). Conclusion Jiawei Danxuan Koukang can improve OSF in rats, which may be related to the increased level of autophagy.
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