高效液相色谱-串联质谱法测定儿童微量脑脊液中美罗培南浓度

doi:10.20047/j.issn1673-7210.2023.22.07

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Abstract Objective To establish a rapid high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the rapid determination of Meropenem in children’s trace cerebrospinal fluid. Methods Using Ertapenem as the internal standard, 10 μl cerebrospinal fluid samples were precipitated by 0.1% formic acid-acetonitrile. The liquid phase separation was performed on a Hypersil GOLDTM column (50 mm×2.1 mm, 3 μm) with 0.1% formic-water and 0.1% formic-acetonitrile as mobile phases， and gradient elution at the flow rate of 0.5 ml/min， and column temperature was 40℃; the detection was carried out by positive electrospray ionization mass spectrometry in multiple reaction monitoring mode. Samples collected from the Department of Neurology of Hebei Children’s Hospital from February 2020 to March 2020 were analyzed. Results The concentration of Meropenem in cerebrospinal fluid was in the range of 0.01-50.00 μg/ml, and the linear equation was Y=0.880X+0.000 168 (r=0.998 9), the lowest detection limit was 0.01 μg/ml; the extraction recoveries were (93.24±9.48)%-(104.62±4.63)%; Meropenem had no significant matrix effect in cerebrospinal fluid. The concentration of Meropenem in cerebrospinal fluid samples was 0.10-1.25 μg/ml. Conclusion The method is sample-saving, simple, rapid, accurate, and sensitive, and is suitable for the concentration monitoring of Meropenem in children’s trace cerebrospinal fluid.

Key words： Meropenem High performance liquid chromatography-tandem mass spectrometry Cerebrospinal fluid Children Trace samples

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	JIANG Xijuan ZHANG Guying YIN Zhiping YANG Jing

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JIANG Xijuan ZHANG Guying YIN Zhiping YANG Jing. Determination of Meropenem concentration in children’s trace cerebrospinal fluid by high performance liquid chromatography-tandem mass spectrometry[J]. 中国医药导报, 2023, 20(22): 34-37.

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https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2023.22.07 OR https://www.yiyaodaobao.com.cn/EN/Y2023/V20/I22/34

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