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| Effects of dipsacus prescription added with Rhizoma Drynariae on bone tissue and PI3K/Akt signaling pathway in rabbits with steriod-induced |
| FU Zixiang WANG Fengming ZHAO Canbin BI Rongxiu LI Xiaoyang |
The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Shandong Province, Jinan 250000, China
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Abstract Objective To observe the therapeutic effect and mechanism of dipsacus prescription added with Rhizoma Drynariae (DPRD) on steroid-induced avascular necrosis of the femoral head(SANFH) rabbits. Methods Twenty-four 2.5-3.0 kg normal grade 4-week-old healthy male New Zealand rabbits were randomly number table method divided into blank group, model group, DPRD low-dose group (group L), and DPRD high-dose group (group H), with six rabbits in each group. The model group, group L, and group H were injected with E. coli endotoxin combined with glucocorticoid for modeling, and MRI was performed six weeks later to evaluate the modeling results. After successful modeling, blank group, and model group were administrated with 5 ml/kg normal saline daily, while group L, and group H were administrated with 2.5 ml/kg and 5.0 ml/kg DPRD decoction, respectively. After six weeks of intervention, MRI was performed, then the animals were killed and the femoral head was removed. The expression levels of phosphatidylinositol 3 kinase (Pi3K), seronine/threonine protein kinase (Akt), phosphorylated protein kinase (P-Akt), human forkhead protein O1 (FOXO1), and vascular endothelial growth factor (VEGF) in the femoral head of rabbits in each group were detected by immunohistochemistry and Western blot. Results After six weeks of modeling, MRI showed that the femoral head of the model group, group L, and group H had collapsed and flattened symptoms, and linear or circular high signal shadow appeared in the femoral head. After six weeks of DPRD intervention, MRI showed that DPRD could effectively improve femoral head necrosis in model rabbits. Western blot and immunohistochemical detection results showed that, compared with blank group, the expression levels of PI3K, Akt, and P-Akt were significantly increased, while the expression level of FOXO1 was significantly decreased in model group (P＜0.05). Compared with model group, the expression levels of PI3K, Akt, and p-Akt in group L and group H were significantly decreased, while the expression level of FOXO1 was significantly increased (P＜0.05). Compared with group L, the expression levels of PI3K, P-Akt, and FOXO1 in group H were increased (P＜0.05), but there was no statistical significance in the expression level of Akt between group L and group H (P＞0.05). In addition, compared with blank group, the expression levels of Akt and P-Akt were increased, and the expression level of VEGF was decreased in model group (P＜0.05). Compared with model group, the expression levels of Akt and P-Akt in group L and group H were decreased, and the expression levels of VEGF were increased (P＜0.05). There were no significant differences in Akt, P-Akt, and VEGF between group L and group H (P＞0.05). Conclusion By regulating PI3K/Akt pathway, DPRD increases the expression of FOXO1, hormone-induced necrosis of femoral head VEGF and reverses the effect of glucocorticoids.
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